基于HPLC-DAD-MS的道地产区大黄药材质量评价研究

Quality evaluation studies on chromatography of Rhei Radix et Rhizoma from geo-authentic producing areas by HPLC-DAD-MS

目的通过对HPLC-DAD-MS多种分析手段的联用,建立大黄药材成分分析方法,确认各个特征峰的归属,从而定性定量的评价药材质量,预测不同道地产区大黄药材的药效,为谱效学研究提供基础研究。方法以全国各道地产区大黄药材为研究对象,采用AgilentZorboxC18(250mm×4.6mm,5μm)色谱柱,乙睛-0.1%磷酸水二元梯度洗脱模式,体积流量为1.0mL/min,检测波长280nm,建立了20批来自四川、青海、甘肃地区的大黄药材的HPLC图谱,并通过HPLC-DAD-MS联用技术对其中30个特征峰进行了归属分类,同时进行了各类指纹成分比较研究。结果各地区间游离蒽醌量差异较小,而其他成分差异明显,表现出可能存在的质量差异。结论本实验全面阐明了大黄药材的各主要化学成分及地区间差异,方法样品处理简单,建立的大黄分析方法稳定性、重复性好,为大黄药材的质量评价提供较为充实的参考依据。

Objective To establish a stable chromatography method of ingredient analysis in the traditional Chinese medicinal materials （TCMM） Rhei Radix et Rhizoma using HPLC-DAD-MS,and identify the attribution of chromatographic peaks.By the chromatography method,the quality of TCMM Rhei Radix et Rhizoma was evaluated by the perspectives of both quality and quantity,and the medicinal effect of Rhei Radix et Rhizoma from different sources was predicted.This study could also provide thoughts and research foundation for chromatographic pharmacodynamics.Methods Agilent Zorbox C18 column （250mm×4.6 mm,5μm）,with mixture of acetonitrile and 0.1% phosphoric acid as mobile phase,flow rate 1.0 mL/min,detective wavelength 280 nm,was used to acquire HPLC chromatogram of 20 Rhei Radix et Rhizoma from different sources in Sichuan,Qinghai,and Gansu provinces.The 30 characteristic peaks were identified and classified using HPLC-DAD-MS and the quantity each various ingredients were compared in fingerprint in this study.Results The results showed that the difference of anthraquinone content from different intervals was not significant,but the significant differences existed in other components,which indicated the quality difference might exist.Conclusion With the simple sample treatment technique,the method of Rhei Radix et Rhizoma established is stable and repeatable,which provides a solid reference for the quality control of Rhei Radix et Rhizoma.