叉头蛋白3在不同分期胃癌组织中的表达

Study on expression of forkhead box protein 3 in different stages of gastric cancer

目的:探讨叉头蛋白3(FoxP3)在胃癌患者病理组织切片中的表达模式。方法:用免疫组化方法对56例不同TNM分期的胃癌患者病理组织进行染色,同时取其中30例患者的正常胃组织作阴性对照,观察FoxP3在胃癌组织中的表达情况,并探讨其表达与患者的性别、年龄、肿瘤TNM分期和转移的关系。结果:胃癌细胞表达FoxP3蛋白,并且主要分布于胃癌细胞的细胞核,少数分布于胞浆区;FoxP3蛋白在胃癌患者中的阳性表达率为68%(38/56),明显高于正常胃组织的23%(7/30),差异具有统计学意义(P<0.05);在有淋巴结转移和无淋巴结转移患者的阳性表达率分别是90%(28/31)和40%(10/25),两者的差异具有统计学意义(P<0.05);FoxP3蛋白表达的阳性率与年龄、性别无关,差异无统计学意义(P>0.05);Ⅲ期、Ⅳ期患者FoxP3表达阳性率明显高于Ⅰ期、Ⅱ期,同时表达量随着TNM分期增高表达逐渐增多,差异具有统计学意义(P<0.05)。结论:高表达的FoxP3可能导致胃癌细胞免疫逃逸参与胃癌的发生、发展过程。

Objective To study the expression of transcription factor forkhead box protein 3（FoxP3）in gastric cancer tissue of patients with different stages of gastric cancer.Methods Sections of gastric cancer tissue of patients with different TNM stages were stained immunohistochemically to study the expression of FoxP3 and their correlations with tumor stage（TNM）,gender,age and metastasis.Results FoxP3 protein was expressed in gastric cancer tissue and was located mainly in nucleus and only few in cytoplasm.The positive rate of FoxP3 in gastric cancer tissue was 68%（38/56）,significantly higher than that in normal tissues 23%（7/30,P0.05）.The positive rates in gastric cancer with and without lymph node metastasis were 90%（28/31） and 40%（10/25）,respectively（P0.05）.The expression of FoxP3 was independent on age or gender（P0.05）.The positive rate of FoxP3 expression in cancer tissue of stage Ⅲand Ⅳ were higher than stage Ⅰ and Ⅱ,and the expression of FoxP3 increased with the increase in TNM stage.Conclusions High expression of FoxP3 may be involved in the immune escape of gastric cancer cell and the process of development and progress of gastric cancer.