兔腹主动脉瘤模型的建立

Establishment of abdominal aortic aneurysm model in rabbits

目的探讨一种简便、成功率高的兔腹主动脉瘤(AAA)模型建立的方法。方法健康雄性新西兰大白兔20只,随机分为实验组和对照组(各10只),实验组应用CaCl2浸润法建模,对照组应用生理盐水浸润法。6周后观察两组动物腹主动脉扩张率、大体形态学及显微病理改变。结果实验组兔腹主动脉直径扩张至(124.12±8.11)%,与对照组相比差异有统计学意义(P<0.01)。实验组腹主动脉的病理改变为中膜弹性纤维组织严重破坏,平滑肌细胞减少。结论 CaCl2溶液浸润法制作兔AAA模型简单可行,为AAA的实验性治疗研究提供一种合适的造模方法。

Objective To investigate a simple and reliable mothod to construction an abdominal aortic aneurysm（AAA） model in rabbits.Methods Twenty male New Zealand rabbits were randomly assigned as experimental group（n=10） and control group（n=10）.AAA model was induced by periarterial infiltration with of calcium chloride（CaCl2） in the experimental group and saline was substituted for CaCl2 in the control group.The dilation ratio,morphological and pathohistological changes of abdominal aorta of the two groups were observed at 6 weeks after operation.Results The dilation ratio of abdominal aorta was（124.12±8.11）% in the experimental group,which was statistically different from that of the control group（P〈0.01）.The pathohistological examination of the abdominal aortas showed serious disruption of the elastic tissue and loss of smooth muscle cells in tunica media of aortic aneurysm in the experimental group.Conclusions The rabbit AAA model using periarterial infiltration with CaCl2 is simple and easy to perform,and it provides an appropriate tool for experimental therapeutic studies in AAA.