阿魏酸对脂多糖诱导的小鼠小胶质细胞炎性反应的抑制作用

Inhibitory effect of ferulic acid on inflammatory response in microglia induced by lipopolysaccharides

阿魏酸是川芎、当归等中药的有效成分之一,具有较强的抗氧化活性和抗炎作用。小胶质细胞是脑内常驻的免疫效应细胞,极易被激活而导致脑内发生慢性神经性炎症反应,与阿尔茨海默病等神经退行性疾病的发生发展密切相关。该研究采用脂多糖(LPS)刺激小胶质细胞(BV-2)活化,研究阿魏酸对炎症反应的抑制作用。结果表明,2.5～22.5μg/mL的阿魏酸浓度依赖性的抑制一氧化氮(NO)、前列腺素E2(PGE2)、白介素-1β(IL-1β)等炎症因子的产生,以及一氧化氮合酶(iNOS)、环氧合酶-2(COX-2)蛋白的表达,其作用机制可能与其抑制Toll样受体4(TLR4)表达有关。

Ferulic acid（FA） is a natural compound that expresses antioxidant and anti-inflammatory activities.Microglial cells are innate immune cells that reside within the central nervous system（CNS）.Activated microglia mediated neuronal immunity contributes to the neurodegeneration associated with Alzheimer＇s disease.In this study,we investigated the inhibitory effect of FA on neuroinflammation in BV-2 microglial cells induced by lipopolysaccharides（LPS）.Our study showed that FA significantly suppressed the production of nitric oxide（NO）,prostaglandin E2（PGE2）,interleukin-1β（IL-1β）,and decreased induced type II nitric oxide synthase（iNOS） and cyclooxygenase-2（COX-2） protein in LPS-stimulated BV-2 microglia cells in a dose dependent manner.We hypothesized that this was achieved by suppressing the protein level of Toll-like receptor 4（TLR4）.