含血栓的曲张大隐静脉管壁肥大细胞的分布与定量研究

Study on distribution and quantitation of mast cell infiltration in vascular wall of varicose veins with thrombus

目的:探讨含血栓的曲张大隐静脉血管壁肥大细胞的浸润情况。方法:收集单纯曲张大隐静脉管壁标本11例为曲张组,含血栓的曲张大隐静脉管壁标本11例为血栓组,另设8例正常大隐静脉管壁标本为对照组,行甲苯胺蓝特殊染色,观察血管壁中肥大细胞浸润的分布与计数情况。结果:血栓组肥大细胞外膜明显增多,呈"聚集征",中膜、内膜呈散在增多;曲张组肥大细胞外膜、中膜、内膜少量散在分布。血栓组管壁肥大细胞总计数与曲张组、对照组之间比较差异有统计学意义(P<0.01),曲张组与对照组之间比较差异无统计学意义(P>0.05)。结论:静脉血栓形成和慢性炎症可能是导致血管壁肥大细胞大量浸润的重要因素。

Objective： To investigate the infiltrating change of mast cell in vascular wall of great saphenous varicose veins with thrombus.Methods： Collecting 11 specimens of simple varicose veins,11 specimens of varicose veins with thrombus and 8 specimens of normal venous tissue were detected the infiltrating distribution and counting of mast cells,with toluidine blue staining.Results： Thrombus group in intima and media,Increased mast cell infiltration was ＇scattered＇,adventitia was ＇gathering sign＇.Varicose group of intima and media,adventitia few scattered mast cells.Thrombus and varicose,control group among the mast cell counting was significantly（P0.01）,varicose and control group showed between no significant difference（P0.05）.Conclusion： Vein thrombosis and chronic inflammation may be important factors that lead to a large number of mast cells infiltration in the vascular wall.