染料介导光氧化处理去细胞牛颈静脉带瓣管道的生物学特性

Biological Characteristics of Decellularized Bovine Jugular Vein Valved Conduit Treated with Dye-mediated Photo Oxidation

目的评价染料介导光氧化处理去细胞牛颈静脉带瓣管道的生物学特性。方法 2009年7月至2010年7月,第四军医大学西京医院采用不同方法处理牛颈静脉带瓣管道,选用年龄2~6岁、体重200~400 kg秦川黄牛的牛颈静脉带瓣管道40根,按随机数字表法分为4组,每组10根。戊二醛处理组（GA组）：用戊二醛处理牛颈静脉带瓣管道;去细胞处理组（DC组）：将牛颈静脉带瓣管道进行去细胞处理;去细胞＋染料介导光氧化联合处理组（DP组）：将牛颈静脉带瓣管道进行去细胞＋染料介导光氧化联合处理;对照组（CO组）：不作固定处理。比较4组牛颈静脉带瓣管道管壁的大体形态、组织学特点、瓣膜厚度、组织含水量、热皱缩温度和断裂强度,并测定可溶性蛋白含量。结果 DP组牛颈静脉带瓣管道的管壁和瓣膜厚度、组织含水量与CO组比较差异均无统计学意义（P〉0.05）,但管壁和瓣膜厚度均小于GA组（管壁：0.8±0.1 mm vs.1.1±0.1 mm,瓣膜：0.2±0.1 mm vs.0.3±0.1 mm,P〈0.05）,管壁和瓣膜组织含水量均大于GA组（管壁：86.1%±2.2%vs.70.4%±2.8%,瓣膜：87.1%±2.5%vs.72.1%±3.1%,P〈0.05）;DP组管壁和瓣膜热皱缩温度、断裂强度与GA组比较差异无统计学意义（P〉0.05）,但管壁和瓣膜热皱缩温度（管壁：84.7±1.4℃vs.70.4±0.3℃,P〈0.05;瓣膜：85.7±1.5℃vs.70.7±0.6℃,P〈0.05）和断裂强度（管壁：10.4±1.1 N vs.6.8±1.0 N,P〈0.05;瓣膜：8.0±0.9 N vs.3.2±0.6 N,P〈0.05）大于CO组。结论去细胞＋染料介导光氧化联合处理牛颈静脉带瓣管道具有较好的生物学特性。

Objective To evaluate the biological characteristics of decellularized bovine jugular vein valved conduit treated with dye-mediated photo oxidation.Methods From July 2009 to July 2010,this study was carried out in Xijing Hospital affiliated to the Fourth Military Medical University.Forty bovine jugular vein valved conduits were obtained from Qinchuan scalpers aged between 2 to 6 years with the weight ranged from 200 to 400 kg.Then,the 40 conduits were divided into four groups by random digital table with 10 in each group.The conduits were treated with glutaraldehyde in the glutaraldehyde group（GA group）,were decellularized in the decellularized group（DC group）,were decellularized and treated with dye-mediated photo oxidation in the decellularized and dye-mediated photo oxidation treated group（DP group）,and were not dealt with in the control group（CO group）.Thickness,appearance,histology,water content,shrinkage temperature,breaking strength,and soluble protein level of the conduit wall and valve were detected and compared among the four groups.Results Thickness and water content of the conduit wall and valve in the DP group were similar to those in the CO group（P0.05）,while thickness of both wall and valve in the DP group was less than those in the GA group（wall： 0.8±0.1 mm vs.1.1±0.1 mm;valve： 0.2±0.1 mm vs.0.3±0.1 mm,P0.05）,and water content of the conduit wall and valve in the DP group was more than those in the GA group（wall： 86.1%±2.2% vs.70.4%±2.8%;valve： 87.1%±2.5% vs.72.1%±3.1%,P0.05）.The breaking strength and shrinkage temperature of the DP group were similar to those of the GA group （P0.05）,while the shrinkage temperature（wall： 84.7±1.4 ℃ vs.70.4±0.3 ℃;valve： 85.7±1.5 ℃ vs.70.7±0.6 ℃,P0.05） and the breaking strength（wall： 10.4±1.1 N vs.6.8±1.0 N;valve： 8.0±0.9 N vs.3.2±0.6 N,P0.05） of the DP group were higher than those in the CO group.Conclusion Decellularized bovine jugular vein valved conduits treated with dye-mediated photo oxidation have nice biological characteristics.