硫化氢对脂肪细胞前体增殖的影响

THE EFFECT OF HYDROGEN SULFIDE ON PROLIFERATION OF 3T3-L1 PREADIPOCYTE

目的:探讨硫化氢对小鼠3T3-L1前脂肪细胞增殖的影响。方法:培养3T3-L1前脂肪细胞,分为对照组、低剂量组和高剂量组(硫化氢浓度分别为0、10、25、50和100μmol/L),采用四甲基偶氮唑盐比色(MTT)法检测硫化氢对3T3-L1前脂肪细胞活性的影响;用BRDU法检测细胞的增殖;结果:与对照组相比,H2S在较高浓度时,对细胞有一定的毒性作用(P<0.05)。结论:硫化氢抑制3T3-L1前脂肪细胞的增殖。

Objective：To disscuss the influence of hydrogen sulfide which actson 3T3-L1 preadipocyte of mouse.Methods：Cul tured the 3T3-L1 preadipocyte,and then divided into three groups：the control group,the low monitoring group and the high monitoring group（hydrogen sulfide density（0μmol/L,10μmol/L,25μmol/L,50μmol/L and 100μmol/L,respectively） using the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide（MTT） colorimetric.Method：to measure the viability of 3T3-L1 preadipocyte to which added hydrogen sulfide.Cell proliferation rate was measured by BrdU.Results： H2S has certain toxic effect on the cell at high concentration compared with the control group（P 0.05）.Conclusion：The hydrogen sulfide inhibits 3T3-L1 preadipocyte＇s proliferation.