髓样细胞表达激发受体-1对急性坏死性胰腺炎大鼠继发感染的判断价值

Diagnostic value of TREM-1 in secondary infection of acute necrotizing pancreatitis of rat

目的探讨髓样细胞表达激发受体-l（TREM-1）在急性坏死性胰腺炎（ANP）大鼠继发感染早期的表达变化及其判断价值。方法24只雄性SD大鼠按数字表法随机分为对照组、ANP组、ANP继发感染（sI）组。通过腹腔注射L一精氨酸、大肠杆菌建模。制模后24h取血和腹水行细菌培养，测血清淀粉酶、C一反应蛋白（CRP）、TNF-α及TREM-1水平，胰腺组织常规病理检查，实时PCR和蛋白质印迹法检测胰腺组织TREM-1mRNA和蛋白的表达。结果ANP组和sI组的胰腺病理评分、血清淀粉酶水平均明显高于对照组，且SI组血和腹水细菌培养阳性率达100％，表明制模成功。sI组血CRP、TNF-α水平分别为（18．7±3．1）mg／L和（185．7±10．9）mg／L，与ANP组的（16．5±3．6）、（176．0±18．6）mg／L无显著差异。sI组的血清TREM-1水平、胰腺组织TREM-1mRNA及蛋白表达量分别为（9．3±0．9）ng／ml、14．84±3．45、316．2±59．2，均显著高于ANP组的（5．5±0．3）ng／ml、4．51±1．44、188．6±42．4（P值均〈0．05）。结论血清TREM-1水平对急性胰腺炎继发感染早期具有一定的判断价值。

Objective To detect the expression of triggering receptor expressed on myeloid cells-1 （TREM-1） in the early secondary infection of acute necrotizing pancreatitis （ANP） and to probe its diagnostic value for early infection. Methods Twenty-four male SD rats were randomly divided into the control （C） group, the ANP group and the secondary infection of ANP （SIANP） group. The constructions of the models were achieved through intraperitoneal injection of L-arginine and E. coli. After 24 hours, the blood and peritoneal fluid samples were collected for bacterial culture, and the serum levels of amylase, CRP, TNF-α and TREM-1 were detected. The pathological changes in the pancreas were observed. The expression of TREM-1 mRNA and TREM-1 protein in pancreatic tissue was detected by Real-time PCR and Western Blot. Results The histological score of pancreas, and serum amylase in ANP group and SIANP group were significantly higher than those in C group; the positive rate of bacterial culture of blood and peritoneal fluid in SIANP group was 100%, which suggested the model was successfully established. CRP and TNF-α levels in SIANP group were （8.7 ±3.1 ） mg/L and （ 185.7 ± 10.9） rag/L, which were not significantly different from that in ANP group [ （ 16.5 ±3.6）, （ 176.0 ± 18.6 ） mg/L]. The serum level of TREM-1, expression of TREM-1 mRNA and TREM-1 protein in pancreatic tissue was （9.3 ± 0.9） ng/ml, 14.84± 3.45, 316.2±59.2, which were significantly higher than those in ANP group [ （5.5 ± 0.3 ） ng/ml, 4.51 ±1.44, 188.6 ±42.4, P 〈 0.05].Conclusions TREM-1 has diagnostic value for early secondary infection of ANP.