HPLC测定3种甘草废渣中光甘草定的含量

HPLC Determination of Glabridin from Three Residues of Glycyrrhiza glabra L. with Different Extraction Methods

目的:测定新疆光果甘草渣、吉尔吉斯光果甘草渣和发霉吉尔吉斯光果甘草渣中光甘草定的含量,比较3种工艺的提取效率。方法:应用闪式法(STE)、超声波法(USE)、微波法(MWE)提取,采用HPLC法测定比较3种甘草废渣中光甘草定的含量。色谱柱条件:KromasilC18色谱柱(4.6mm×150mm,5μm),流动相:乙腈-1%醋酸水溶液(53:47),流速:1mL.min-1;检测波长:282nm;柱温:25℃。结果:不同甘草废渣中光甘草定的含量不同,3种提取工艺光甘草定含量有差异。结论:在本试验条件下,吉尔吉斯甘草废渣中光甘草定含量较高;霉变的光甘草定含量较低。STE法光甘草定提取率较高。

Objective： To determine the glabridin content in 3 kinds of residues of Glycyrrhiza glabra L. （licorice） and compare the extraction yields with different methods. Mehtods： Smashing tissue extraction （STE）, ultrasound-assisted extraction （USE） and microwave-assisted extractions （MWE） were used for extracting glabridin and the content of glabridin were determinated by HPLC method. The Kromasil Cls column （4.6 mm × 150 mm, 5 μm） was used. The mobile phase was acetonitrile-water（ containing 1% acetic acid） （53：47 ）. The detection wavelength was 282 nm. The column temperature was 25 ℃. Results： The glabridin, content in three licorice residues with three different methods for extracting glabridin are different. Conclusion： The glabridin content from Kirghizia licorice is higher and that in mildewed Kirghizia licorice decreased. Among three extraction methods, the glabridin content extracted with STE is relatively higher in the test conditions.