CP慢性染毒对大鼠脑皮层乙酰胆碱酯酶活性的影响

Effect of Chronic Exposure to CP on AChE Activity of Cerebral Cortex in Rats

为探讨CP慢性染毒对大鼠脑皮层乙酰胆碱酯酶活性的影响,将120只雄性大鼠随机分为4组,即对照组(花生油)、低剂量组(40mg/kgCP)、中剂量组(80mg/kgCP)和高剂量组(160mg/kgCP),每组30只.灌胃给药28次,每日1次,共28d,每日观察动物症状.给药14d、28d和给药结束后7d各组分别选择10只动物,处死动物分离脑皮层,免疫组化测定AChE活性.结果显示:CP慢性染毒后大鼠表现出神经受损症状,低、中和高剂量组皮层AChE活性均显著降低(P<0.05),并呈剂量依赖性.结果表明:神经损伤大鼠脑皮层AChE活性显著降低,AChE活性具备成为神经损伤标志物的潜力.

To study the effect of chronic exposure to CP on AChE activity of Cerebral Cortex in Rats,120 male SD rats were equally divided into 4 groups,including control group（peanut oil）,low dosage group（40 mg cp/kg body）,middle dosage group（80 mg cp/kg body） and high dosage group（160 mg cp/kg body）.Intragastric administration at 1 time a day were planed,the treatment lasted 28 days.General symptoms of rat were observed everyday.10 rats of each group were selected at 14,28 day after treatment and 7 days after last treatment,respectively,cerebral cortex of selected rats were separated,AChE activity were measured by immunohistochemistry.Results showed that rats displayed symptoms of nerve damage,AChE activity of cerebral cortex of 3 administration were all decreased significantly（P0.05）,dose-dependent was found.Results indicated AChE activity of cerebral cortex were decreased significantly in nerve damage rats,AChE activity may be treated as marker of nerve damage.