紫杉醇对TGF-β_1促人Tenon’s囊成纤维细胞增殖的影响

Effect of paclitaxel on proliferation of human Tenon’s capsule fibroblasts stimulated by TGF-β_1

目的:研究转化生长因子-β1(transforming growth factor-β1,TGF-β1)促人Tenon’s囊成纤维细胞(human Tenon’s cap-sule fibroblasts,HTFs)的增殖作用及紫杉醇对该增殖作用的影响。方法:用TGF-β1(0～50 ng/ml)作用于体外培养的HTFs,CCK-8法检测其增殖;用紫杉醇(0～10 000 nmol/L)作用于HTFs 2～24 h,Hochest 33342染色检测其凋亡。结果:2.5～25.0 ng/ml TGF-β1作用2～24 h均可以促进HTFs增殖,10～10 000 nmol/L紫杉醇能抑制TGF-β1促HTFs的增殖作用,其抑制作用与浓度成正比;Hochest 33342染色证实10～10 000 nmol/L紫杉醇对于增殖的HTFs有促凋亡的作用并与浓度成正比。结论:TGF-β1可刺激HTFs增殖,紫杉醇可抑制TGF-β1促HTFs的增殖作用,可以促进细胞的凋亡。

Objective：To investigate the effect of TGF-β1 on the proliferation of human Tenon’s capsule fibroblasts（HTFs） and the effect of Paclitaxel on the proliferation of HTFs stimulated by TGF-β1.Methods： ① HTFs cultured by tissue block adhering wall method were observed by optical microscope and identified by immunocytochemistry methods.② The 4th and 6th generation of HTFs were in-cubated with various concentrations of TGF-β1（0,2.5,5,10,25,50 ng/ml）for 24 hours.The proliferation of HTFs was evaluated by CCK-8.③HTFs were treated with 10 ng/ml TGF-β1 and various concentration of Paclitaxel（0,10,100,1 000,10 000 nmol/L） for 24 h in order observe proliferation.④ The cell apoptosis was measured by Hochest33342 staining.Results：① The Tenon’s fibroblasts were successfully cultured.The results of vimentin immunocytochemisty staining was positive.② Treatment of the cells with 2.5～25.0 ng/ml TGF-β1 increased the proliferation of the HTFs.③ 100～10 000 nmol/L Paclitaxel suppressed the proliferation of the HTFs stimulated by TGF-β1.④ Hochest staining shows a clear apoptotic features.Paclitaxel treatment resulted in increased number of apoptotic cells in a dose-dependent manner.Conclusion： TGF-β1 may increase the proliferation of HTFs,which could be reduced by Paclitaxel.Pacli-taxel could bind to TGF-β1 receptor and interfere in signal transmission and inhibit the scarring after glaucoma filtering surgery.