摘要
目的:调查江苏骨髓库志愿者HLA-B*15等位基因的分布特征,研究其可能对临床供体选择的影响以及相关疾病。方法:利用聚合酶链反应-碱基序列直接测序(PCR-SBT)方法对3 238例江苏人群HLA-B位点的2、3、4外显子序列进行分型,计算HLA-B*15等位基因频率,并与不同人群进行比较。结果:本研究中检出的21种HLA-B*15等位基因有:B*1501(B62),B*1502(B75),B*1503(B72),B*1505(B62),B*1507(B62),B*1508(B75),B*1510(B71),B*1511(B75),B*1512(B76),B*1513(B77),B*1517(B63),B*1518(B71),B*1521(B75),B*1525(B62),B*1527(B62),B*1529(B15),B*1532(B62),B*1535(B62),B*1546(B72),B*1558(B62),B*1568(B35)。其中以B*1501(4.69%)最常见,其次为B*1511(2.55%),B*1502(2.10%),B*1518(1.34%),B*1527(1.27%)。结论:在江苏汉族人群中HLA-B*15等位基因水平表现出丰富的多态性,利用其分布特点,为临床移植供者选择和HLA相关疾病分析提供了依据。
Objective:To investigate the characteristics of the allele distribution of HLA-B^*15 gene family in Jiangsu population,then to study its influence on the selection of clinical transplantation donor and HLA related diseases.Methods:A total of 3 238 cord blood samples from Jiangsu Cord Blood Bank were analyzed by sequence-based typing.Then the sequences encompassing exon 2,3,and 4 for HLA-B gene were typed by direct sequencing of PCR products.The gene frequencies distribution of HLA-B in this popula-tion was compared with that in other populations.Results:A total of 21 different HLA-B^*15 alleles were detected: B^*1501(B62),B^* 1502(B75),B^*1503(B72),B^*1505(B62),B^*1507(B62),B^*1508(B75),B^*1510(B71),B^*1511(B75),B^*1512(B76),B^*1513(B77),B^*1517(B63),B^*1518(B71),B^*1521(B75),B^*1525(B62),B^*1527(B62),B^*1529(B15),B^*1532(B62),B^*1535(B62),B^* 1546(B72),B^*1558(B62) and B^*1568(B35).A total of 21 different HLA-B^*15 alleles were identified.B^*1501(4.69%) was the most frequent allele,followed by B^*1511(2.55%),B^*1502(2.10%),B^*1518(1.34%),B^*1527(1.27%).Conclusion:Our study demonstrates the high diversity of HLA-B^*15 in the Jiangsu population,which shows direct significance for the selection of trans-plantation donor,the diagnosis and treatment the HLA related diseases.
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
北大核心
2011年第6期813-817,共5页
Journal of Nanjing Medical University(Natural Sciences)