二氧化氯对乙型肝炎表面抗原灭活效果的研究

Effects of Chlorine Dioxide on Inactivation of Hepatitis B Virus Surface Antigen

为探讨二氧化氯（ＣｌＯ２） 的消毒效果，应用酶联免疫吸附实验（ ＥＬＩＳＡ） ，研究了ＣｌＯ２ 对乙型肝炎病毒表面抗原（ ＨＢｓＡｇ） 的灭活效果并与液氯（Ｃｌ２） 进行对比。结果表明：２－５ ｍ ｇ／Ｌ 的ＣｌＯ２ 可使５８－４ ％ 的ＨＢｓＡｇ 灭活，１２－５ ｍｇ／Ｌ 的Ｃｌ２ 可灭活５２－２ ％ 的ＨＢｓＡｇ ；在不同的ｐ Ｈ 条件下，ＣｌＯ２ 对ＨＢｓＡｇ 的灭活效果都高于Ｃｌ２ ，在ｐＨ ＝ ７ 消毒效果最好；２５ｍｇ／Ｌ ＣｌＯ２ 作用２０ ｍｉｎ 可使９７－２ ％ 一定浓度的ＨＢｓＡｇ 灭活。而２５ ｍ ｇ／Ｌ Ｃｌ２ 经过６０ ｍｉｎ 只能使７０－２ ％ 相同浓度的ＨＢｓＡｇ 灭活，说明ＣｌＯ２ 是一种高效。

To study the disinfective effects of chlorine dioxide (ClO 2),the effects of ClO 2 on inactivation of hepatitis B virus surface antigen (HBsAg) were determind by the method of enzyme linked immunosorbent assay(ELISA) and were compared with those of chlorine(Cl 2).The results showed that 2.5 mg/L ClO 2 could inactivate 58.4% of HBsAg,while 12 5 mg/L Cl 2 could inactivate only 52.2% of HBsAg;the effects of ClO 2 on inactivation of HBsAg were better than those of Cl 2 with different pH values,it reached to the best effect at pH=7 97.2% of HBsAg with a certain concentration could be inactiveated by an exposure to 25 mg/L ClO 2 for 20 minutes,and only 70.2% of HBsAg with a same concentration could be inactivated by an exposure to 25 mg/L Cl 2 for 60 minutes.It indicated that ClO 2 was an effective,rapid disinfectant for drinking water.