摘要
采用SD 大鼠离体气管,与重金属解毒药二巯基丁二酸( DMSA) 对照,观察了解毒健脾中药提取物02 号( 中药02 号) 对呼吸道平滑肌镉染毒的保护作用。结果显示:(1) 镉对气管平滑肌的抑制作用在一定范围内呈浓度依赖性。当氯化镉浓度为6 m mol/L,9 m m ol/L 时,气管平滑肌对乙酰胆碱的最大收缩效应(Emax) 从98 ±3( n = 6) 分别降至53 ±28( n = 7 ,P< 0-05) 、19 ±15( n = 7 ,P< 0-001 ;(2) 当用中药02 号预处理气管后,氯化镉的抑制作用减弱,Emax 从19 ±15 恢复至62 ±27( n = 5 ,P< 0-05) 和56 ±28( n = 5 ,P< 0-05) 与用DMSA 后(Em ax 恢复至40 ±10 和70 ±10) 比较,两者间无显著性差异( P> 0-05) 。氯化镉对气管平滑肌收缩的抑制作用,可能与其影响乙酰胆碱对M 受体的亲和力和( 或) 抑制钙通道有关;中药02 号的作用,可能与DMSA 的解毒机理相同,即与氯化镉形成络合物,而发挥预防作用。
Compared with acid dimercaptosuccinate(DMSA),the preventive effect of extract extracted from a Chinese formula“N 02 Detoxifying and Spleen strengthening”(N 02),was studied on the isolated SD rat tracheal smooth muscle pretreated with cadmium chloride(CdCl 2).The data showed that:(1)Cd dose dependently inhibited the contracted effect of rat tracheal smooth muscle in certain range of concentrations.At the concentrations of 6 mmol/L and 9 mmol/L of Cd,the maximal effect(Emax) of strips to acetylcholine(ACh) significantly decreased from 98±3( n=6) to 53±28( n=7,P<0 05) and 19±15( n=7,P<0 001) ,respectively;(2)After pretreatment with N 02,the inhibition of Cd to the contracted isolated strips was significantly reduced and the Emax was reestablished to 62±27( n=5,P<0.05 ) and 56±28( n=5,P<0 05) ,respectively,which showed no significant difference with that of DMSA(40±10 and 70±10, P>0 05) ,The inhibition effect of Cd on the contracted airway smooth muscle would be concerned in Cd's influence on affinity of muscarinic receptors to ACh and(or) in its inhibition on the calcium channel.The mechanism of preventive effect of N 02 was probably similar to that of DMSA,forming the chelate with Cd.It indicated that traditional chinese medicine might be a new way to prevent or treat cadmium poisoning.
出处
《环境与健康杂志》
CAS
CSSCI
CSCD
北大核心
1999年第6期343-345,共3页
Journal of Environment and Health
基金
国家自然科学基金
关键词
镉中毒
乙酰胆碱
抑制
预防作用
中药
Cadmium Acetylcholine Trachea smooth muscle Inhibition Preventive effect Traditional Chinese medicine Acid dimercaptosuccinate