辛伐他汀对蛛网膜下腔出血大鼠海马TNF-α的影响

EFFECT OF SIMVASTATION ON TUMOR NECROSIS FACTOR-ALPHA IN HIPPOCAMPUS OF SUBARACHNOID HEMORRHAGE MODEL RATS

目的探讨辛伐他汀对蛛网膜下腔出血大鼠海马肿瘤坏死因子-α(TNF-α)的影响。方法将SD大鼠分为对照组、模型组和治疗组,采用非开颅血管内穿线法制备大鼠SAH模型,治疗组给予辛伐他汀。采用免疫组织化学和免疫印迹方法检测大鼠海马TNF-α表达。结果模型组神经功能缺损明显,海马TNF-α表达明显高于对照组(P<0.05)。使用辛伐他汀后,TNF-α表达降低(P<0.05)。结论 TNF-α参与了蛛网膜下腔出血发病过程,辛伐他汀可能通过影响TNF-α途径发挥治疗蛛网膜下腔出血脑血管痉挛作用。

Objective To observe the effect of simvastatin on tumor necrosis factor-alpha（TNF-α） in hippocampus of subarachnoid hemorrhage（SAH） model rats.Methods Thirty Sprague Dawley（SD） rats were divided into three groups randomly.Control group：only cistema magna puncture was done with no injection of blood.Model group：SAH model by endovascular perforation without opening cranium,Treatment group： rats were subcutaneously injected daily with simvastatin（20mg/kg,0.1ml） for 7 days after the first puncture.Expression of TNF-α protein in hippocampus were detected by immunohistochemistry and western-blotting.Results Compared with the control group,increased expression of TNF-α was detected in model group（P0.05）.While lower expression of TNF-αwas detected in treatment group（P0.05）.Conclusion Simvastatin treatment after SAH attenuated neurological deficits in rat.The mechanism may be attributable partly to TNF-α down regulation.