水稻内稃扭曲突变体palea distortion 1(pd1)的鉴定与基因定位

Identification and Gene Mapping of a palea distortion 1(pd1) Mutant in Rice (Oryza sativa L.)

在对籼稻缙恢10进行EMS(ethyl methane sulfonate)处理后的群体中,发现一个花器官突变体,主要表现为内稃扭曲并呈现外稃化特征,浆片数目增加且呈现稃状特征,雄蕊数目减少至1～4个,部分雄蕊的花丝呈现浆片化特征,暂将其命名为水稻颖壳扭曲突变体palea distortion 1(pd1)。遗传分析表明该突变性状受一个隐形单基因控制。利用群体分离分析法(bulked segregation analysis,BSA),将PD1基因定位在第2染色体的RM13693和RM13936之间,遗传距离分别为3.25cM和3.90cM。该研究结果为PD1基因的图位克隆奠定了基础。

A new floral organ mutant was found in the group which dealt with ethyl methane sulfonate in indica rice-Jinhui10. In the mutant flowers, palea was distorted and showed lemma-like identity, lodicules was transformed into glume-like organs with the increase of number, the number of stamens was reduced to 1 to 4, and the filament of some stamens showed lodicule-like identity. We temporarily designate it as palea distortion 1（pd1） mutant. Genetic analysis indicated the pd1 trait was controlled by a single recessive gene. By bulked segregation analysis （BSA） and rice SSR molecular maker, the PD1 gene was preliminary located between RM13693 and RM- 13936 on chromosome 2, with genetic distances 3.25 cM and 3.90 cM, respectively. This result provided a foundation of map-based cloning and function analysis of PD1 gene.