胸腺素α原作为约氏疟原虫疫苗免疫佐剂的初步研究

Study on the prothymosin α as vaccine adjuvant of P.yoelii

目的:研究胸腺素α原(Prothymosinα,ProTα)作为约氏疟原虫疫苗免疫佐剂的作用。方法:提取P.yoelii-17XNL全蛋白作为抗原,用胸腺素α原作为免疫佐剂,免疫小鼠。具体方案为:昆明小鼠分为4组,每组6只,A组免疫P.yoelii-17XNL全蛋白+ProTα;B组免疫P.yoelii-17XNL全蛋白;C组只注射ProTα;D组为空白对照,以相同体积的生理盐水代替。免疫结束后感染致死的P.yoelii-17XL,1×107个虫/只小鼠。结果:感染后的前10天A组小鼠疟原虫血症平均值要低于其他三组,且最终有3只小鼠存活下来,存活率50%,C组有一只小鼠存活,B、D组小鼠全部死亡。结论:用P.yoelii-17XNL全蛋白做抗原,用ProTα作为佐剂,比单独用P.yoelii-17XNL全蛋白对小鼠有更好的免疫保护作用,提示了ProTα可以成为一种有潜力的蛋白疫苗。

Objective：To investigate the function of prothymosin α（ProTα） as vaccine adjuvant of P. yoelii. Methods： The mice were immunized with the total protein extracted from P. yoelii-17XNL as antigen, together with prothyinosin α as adjuvant. Programs： Kurnming mice were divided into A, B, C and D group. A group was immunized with P. yoelii-17XNL total protein and ProTa; B group was immunized with P. yoelii-17XNL; C group was only injected with ProTα; D group was the control, only injected with physiological saline. And then, the mice of each group was infected with P. yoelii-17XL, the dosage was 1 × 107/mice. Results： The pmrasitemia of A group-mice was lower than the other three groups in the first 10 days after infection, and eventually there were three mice survived in A group, the survival rate was 50%, one mouse survived in C group, all of mice in B group and D group died. Conclusion： Mice immunized with P. yoelii-17XNL total protein as antigen together with ProTα as adjuvants, had better immune protection than those immunized with P. yoelii-17XNL protein only. The present results suggest that the ProTα can act as a potential adjuvant in protein vaccine.