大豆未成熟种子子叶节不定芽再生的初步研究

Shoots Regeneration of Cotyledon Node from Soybean Immature Seeds

以冀豆12和中黄13的未成熟种子子叶节为外植体,研究了6-BA浓度、取材时间和种子发育程度对大豆未成熟种子子叶节不定芽诱导的影响。结果表明:冀豆12最佳取材时间为开花后60～66 d,最佳6-BA浓度为1.0～1.5 mg.L-1,出芽率达96.67%～100%。中黄13最佳取材时间为开花后49～65 d,6-BA浓度为1.5 mg.L-1,出芽率达93.33%～100%。冀豆12和中黄13未成熟种子子叶节的出芽率比成熟种子子叶节分别提高4%和10%。

Immature seed cotyledons nodes of Jidou 12 and Zhonghuang 13 were used as explants to research the effect of 6-BA concentration,time of obtaining materials and degree of seeds development on the proportion of shoots regeneration from immature seeds cotyledons nodes.The result showed that the best time of obtaining materials of Jidou 12 was 60-66 d after flowering,the optimal concentration of 6-BA in multiple shoots induction medium was 1.0-1.5 mg·L^-1and the proportion of shoots reached 96.67%-100%.The best time of obtaining materials of Zhonghuang 13 was 49-65 d after flowering,the optimal concentration of 6-BA in multiple shoots induction medium was 1.5 mg·L^-1and the proportion of shoots reached 93.33%-100%.Proportion of shoots by immature seeds cotyledons nodes were higher than mature seeds cotyledons nodes by 4%and 10%for Jidou 12 and Zhonghuang 13,respectively.