摘要
利用60对SSR引物对东北52份野生大豆资源进行遗传多样性分析。结果表明:①其中40对SSR引物扩增出408个等位变异,平均每个位点等位变异10.2个,Shannon-Weaver指数变化范围为1.2203～2.6392,平均为2.0560,东北野生大豆具有较丰富的遗传变异;②41～43°N×124～126°E区的野生大豆资源分布广、遗传多样性丰富,推测处于该经纬区间的辽河平原为东北野生大豆的初生遗传多样性中心;39～41°N×120～122°E区的野生大豆遗传多样性指数较高,但搜集的资源仅有4份,推测处于该经纬区间的辽东丘陵区可能为东北野生大豆的次生多样性中心。证明东北野生大豆形成了不均衡的2个多样性中心;③基于SSR数据,把52份野生大豆材料共聚为6大类,聚类结果与野生大豆材料地理分布具有一定的相关性。
The genetic diversity of 52 northeast wild soybeans were analyzed by 60 pairs of SSR primers.The results showed that： 408 alleles were detected by 40 pairs of SSR markers,with an average of 12.5 alleles for each locus.Shannon-Weaver diversity indexes varied from 1.2203 to 2.6392 with averaged 2.0560,wild soybean has a rich genetic variation in northeast China;wild soybean in 41-43°N×124-126°E area have the richest accessions and genetic diversity,thus the Liaohe plain might be the newborn genetic diversity centre.Wild soybean in 39-41 °N × 120-122 °E region have higher genetic diversity index,but fewer resources were collected,thus the Liaodong hilly area might be the secondary genetic diversity centre of the northeast China.It proved that the formation of two uneven wild soybean centers of diversity in northeast China.Based on SSR data,52 wild soybeans were clustered into six categories,the results of clustering was related with the geographical distribution of wild soybeans.
出处
《大豆科学》
CAS
CSCD
北大核心
2011年第3期388-392,396,共6页
Soybean Science
基金
辽宁省高等学校创新团队科研资助项目(2009T088)
