大豆子叶节及苗期NaCl筛选试验研究

Susceptibility of Soybean Cotyledonary Node and Seedlings to NaCl Stress

以NaCl为筛选剂,对5个大豆品种的子叶节诱导、丛生芽生根及大豆苗期的生长情况进行研究。结果表明:不同基因型大豆对NaCl的敏感性不同,同一基因型的不同部位对NaCl的敏感性也存在差异。最终确定了NaCl对子叶节分化、丛生芽生根以及苗期生长适宜的筛选浓度。大豆子叶节分化NaCl临界筛选浓度:吉农27和吉农17为225 mmol.L-1;吉林30和DE2259为200 mmol.L-1;吉农28为175 mmol.L-1。丛生芽生根NaCl临界筛选浓度:吉农27和吉农17为100 mmol.L-1;吉林30、DE2259和吉农28为75 mmol.L-1。大豆苗期NaCl临界筛选浓度:吉农27、吉农17、吉林30和DE2259均为250 mmol.L-1;吉农28为200 mmol.L-1。

During plant genetic transformation, the use of marker gene is an efficient method to select and identity transgenic plants. Using safe selectable marker not only screened transformants but also decreased potential risk of genetically modified food. In this study, NaCI was regarded as selectable marker. The effects of NaCl on cotyledonary node bud differentiation, rooting of shoots and growing of soybean seedling were investigated using five soybean varieties. The results showed that the effects of NaCl on different genotypes and explants exist differences. For cotyledonary node, the critical concentration of NaCl was 225 mmol·L^-1 for Jinong 27 and Jinong 17; 200 mmol·L^-1 for Jilin 30 and DE2259; 175 mmol·L^-1 for Jinong 28. For rooting, the critical concentration of NaCl was 100 mmol·L^-1 for Jinong 27 and Jinong 17; 75 mmol·L^-1 for Jilin 30, DE2259 and Jinong 28. For soybean seedling the critical concentration of NaCl was 250 mmol·L^-1for Jinong 27, Jinong 17, Jilin 30 and DE2259 ; 200 mmol·L^-1 for Jinong 28.