新聚丙烯酰胺凝胶电泳快速检测大豆脂氧酶缺失方法

A Fast Detection Method for Screening of Lipoxygenase Null Individual Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis in Soybean

通过对传统SDS-PAGE电泳技术中样品前处理及制胶技术的改进,开发出了一种能够清晰鉴别Lox-1与Lox-2同工酶的SDS-PAGE电泳快速检测新技术。利用新改进的方法,在289个CS8000(♀)×FJ307(♂)F2杂交后代中,选拔出Lox-1,-2,-3同工酶完全缺失的个体27株,以及Lox-1,-3和Lox-2,-3缺失中间材料若干株。证明新改进方法可以缩短杂交后代脂氧酶缺失个体筛选进程,提高筛选精度,降低筛选成本。

In this research,through the improvement of the sample pretreatment and confection of the gel,a fast detection method were developed for screening of lipoxygenase（Lox） null individual using sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE） in soybean.By this method,we have screened 27 strains of the Lox-1,-2,-3 null,and some Lox-1,-3 or Lox-2,-3 incompletion lacking individuals from 289 individuals in the F2（CS8000（♀）×FJ307（♂））generations.The results proved that the method was better in precision,velocity and less costs of the screening.It is significant for selecting the lipoxygenase null individual in quality improvement breeding of soybean.