骨金散对去卵巢大鼠骨密度、雌激素水平的影响

Effects of Gujinsan on bone mineral density(BMD) and estrogen(E_2) level in ovariectomized rats

目的:复制去卵巢大鼠骨质疏松的动物模型,探讨中药骨金散对去卵巢雌性大鼠雌激素和骨密度的影响。方法:6月龄雌性SD大鼠40只,随机分为去势组和假手术组。去势组切除双侧卵巢,假手术组切除部分脂肪。大鼠去势后1个月,将去势组随机分为3组:模型组、雌激素组和骨金散组,雌激素组每日灌服己烯雌酚0.05mg/kg,骨金散组按1.8g/kg剂量灌服骨金散,其余各组灌服等量生理盐水。2个月后,酶联免疫吸附法测量雌激素(E2)水平,双能X线骨密度仪测定离体左右肢股骨、胫骨骨密度。结果:与假手术组相比,模型组体重明显升高(P<0.01),骨密度降低和E2水平显著下降(P<0.01),骨金散组体重明显低于模型组(P<0.01),骨密度、E2水平明显高于模型组(P<0.05)。结论:增加去势大鼠BMD、血清E2水平,提示这一作用是骨金散治疗骨质疏松的机理之一。

Objective：To establish the animal models of osteoporosis in ovariectomized rats and investigate the effects of Gujinsan on bone mineral density（BMD） the level of estrogen（E2） in ovariectomized rats.Methods：40 six-month-old Sprague-Dawley female rats were randomly assigned to two groups,including ovariectomized group and false operation group.In the ovariectomized group,double ovaries of rats were removed.In the false operation group,only the fat of rats were exposed.At 1 month after operation,the ovariectomized group were randomly allocated into ovariectomized control group,estrogen group,Gujinsan group,and received medicine intervention as follows： estrogen group： intragastric administrated stilboestrol（0.05mg/kg per day）;Gujinsan groups： intragastric administrated Gujinsan（1.8g/kg per day）;the rest of groups：intragastric administrated the same amount of normal saline.After 2 months,serum E2 was detected by using enzyme linked immunosorbent assay（ELISA） and BMD was measured by using double functional X-ray digital bone density meter.Results：Compared with false operation group,the body weight of ovariectomized control group was significantly increased（P〈0.01）,and that of ovariectomized control group was also significantly increased compared with that of estrogen group and Gujinsan group（P〈0.01）.BMD and serum E2 of ovariectomized control group were decreased significantly（P〈0.01）,while compared with ovariectomized control group,BMD and E2 of estrogen group and Gujinsan group were increased significantly（P〈0.05）.Conclusion：Gujinsan can increase BMD and serum E2 in ovariectomized rats,which may be one of the mechanisms of Gujinsan in the treatment of osteoporosis.