液基细胞中p^(53)异常表达在宫颈鳞癌诊断中的应用价值

Evaluation of p^(53) in LCT in squamous cell carcinoma

目的:研究液基细胞(LCT)中p53异常表达在宫颈癌诊断中的应用价值。方法:采用免疫细胞化学法分别检测p53基因在41例宫颈鳞状上皮癌(SCC)组和35例正常宫颈(NILM)组中的表达情况。结果:p53基因表达在宫颈鳞状上皮癌的表达率为58.54%,正常组的表达率为5.71%,两组之间存在明显差异(P<0.05)。结论:采用液基细胞学联合p53免疫细胞化学染色方法可提高宫颈癌病变的敏感度和特异性,可作为检测宫颈癌的辅助诊断方法。

Objective：To investigate the value of tumor marker p^53 in LCT in squamous cell carcinoma.Methods：A total of 76 cytology specimens were analyzed,including 41 squamous cell carcinomas（SCC） and 35 cases of NILM within normal limits.LCT was used in all the cases.Immunocytochemistry and immunohistochemistry were used to detect the expression of p^53.Results：The positive rates of p^53 in SCC group and NIML group were 58.54% and 5.71%.,with significant difference in NIML and SCC between the two groups.Conclusion：LCT method and the combination of LCT with p^53 immunocytochemistry can be used for the diagnosis of early cervical cancer.