蛇床子素对乳鼠心肌细胞钠通道的影响

Effect of Osthole on Sodium Current in Rat Ventricular Cardiomyocytes

目的:研究蛇床子素对体外培养大鼠乳鼠心肌细胞钠通道电流的影响。方法:采用全细胞膜片钳技术,首先在乳鼠心肌细胞上诱导出钠电流,然后观察蛇床子素对钠电流的影响。结果:蛇床子素可以浓度依赖性抑制心肌细胞钠通道,抑制的半有效浓度为110.3±6.5μM。蛇床子素对钠电流的激活阈电位和峰电位没有影响,但是其对钠电流的抑制表现出电压依赖性。蛇床子素使钠通道稳态失活曲线向超极化方向漂移。此外,蛇床子素对钠电流的抑制是快速可逆的,这种抑制具有使用依赖性。结论:蛇床子素以浓度依赖和使用依赖的方式抑制乳鼠心肌细胞钠通道电流。

Objective：To investigate the effects of osthole on voltage-dependent sodium currents in rat ventricular cardiomyocytes in vitro.Methods：The sodium currents were recorded in rat ventricular cardiomyocytes using whole cell patch clamp technique.The effects of osthole on sodium currents were investigated.Results：Osthole could inhibit sodium currents in a concentration-dependent manner.The half-effective concentration of the inhibition was 110.3 ± 6.5μM.Osthole showed no influence on the activation threshold and peak voltage of the sodium channels.However,the inhibition by osthole was voltage-dependent.Osthole shift the steady-state inactivation curve in a hyperpolarized direction.The inhibition by osthole was fast and reversible.Furthermore,the inhibition by osthole was use-dependent.Conclusion：Osthole decreased sodium currents in rat ventricular cardiomyocytes in concentration-dependent and use-dependent.