低频振动对BMSCs成骨能力影响及机制的在体实验研究

Influence of low frequency vibration on osteogenesis of bone marrow stromal cells and its mechanism in vivo experiment

[目的]通过在体实验,研究低频振动对骨髓基质干细胞(bone marrow stromal cells,BMSCs)成骨能力及其OPG基因、RANKL基因表达的影响,并初步探讨其机制。[方法]实验用新西兰兔80只,制作骨缺损模型,骨缺损区植入脱钙骨基质明胶及BMSCs复合物,随机分组:对照组(A组);12.5 Hz振动频率组(B组);25 Hz振动频率组(C组);50 Hz振动频率组(D组);100 Hz振动频率组(E组)。振动组于第7 d开始接受振动干预5周;振动结束后对不同频率振动组别OPG mRNA、RANKL mRNA进行检测。[结果]振动组BMSCs OPG、RANKL基因表达明显上调(P<0.05),以25 Hz显著(P<0.01);100 Hz振动组BMSCs OPG、RANKL基因表达下调(P<0.05)。[结论]低频振动可调控BMSCs的骨向分化并且促进其成骨能力,可能与其促进OPG基因表达上调有关。

[ Objectivel To explore the effect of low frequency vibration on osteogenesis of bone marrow stromal cells （BMSCs） and OPG mRNA, RANKL mRNA expression through the vivo experiment. [ Methods] Eighty experimental rabbits were randomly divided into control group （A group）, 12. 5Hz vibration frequency group （B group）, 25Hz vibration frequency group （ C group）, 50Hz vibration frequency group （ D group） and lOOHz vibration frequency group （ E group） . At 7 days, vibration groups B, C, D and E received low frequency vibration for 5 weeks. After vibration , OPG mRNA, RANKL mRNA were detected. [ Results ] In the vibration groups OPG and RANKL gene expressions were significantly increased （ P 〈 0. 05 ）, particularly in 25Hz group （P 〈 0.01 ）, but decreased in lOOHz group （ P 〈 0. 05 ） . [ Conclusion] Low frequency vibration can control the differentiation of BMSCs and promote their osteogenic capability, which is attributed to the elevated OPG gene expression.