人参皂甙Rh2和桦木酸协同诱导A549细胞凋亡的研究

Synergism of ginsenoside Rh2 and betulinic acid to induce apoptosis in A549 cells

我们已经发现,天然成分人参皂甙Rh2(G-Rh2)和桦木酸(Bet A)能够协同作用诱导人源肺癌A549细胞死亡.通过细胞形态观察、流式细胞检测分析、酶活性测定、免疫印记分析等实验技术,从细胞和分子水平提供证据,证明了G-Rh2和Bet A协同诱导A549细胞死亡的过程为细胞凋亡作用,且涉及线粒体途径.

In previous study,we showed that two natural compounds ginsenoside Rh2（G-Rh2） and betulinic acid（Bet A） synergistically induce cell death in human lung cancer A549.In this paper,using cell morphology assay,flow cytometry analysis,enzyme activity assay,and immunoblotting analysis,we demonstrated that G-Rh2 and betulinic acid synergistically induce mitochondrial pathway associated apoptotic cell death in A549 cells.