春尺蠖核型多角体病毒人工扩繁技术研究初报

Artificial mass propagation techniques of Apocheima cinerarius NPV

利用自制的扩繁饲养棚开展春尺蠖Apocheima cinerarius Erschoff幼虫饲养、接毒增殖、采集回收、加工配制、储藏保存等一系列技术研究,结果表明,室内配比成虫平均产受精卵153粒;孵化率为86.9%;幼虫存活率70%;接毒幼虫感病率92.9%;回收率82.1%;在-18～3℃条件下冷藏保存2 a的病毒制剂毒力仅衰减1%。

The techniques such as the rearing of Apocheima cinerarius larvae, inoculation and propagation, collection and recovery, processing and preparation, storage and preservation were studied in the self made net-houses. The results showed that the average number of fertilized eggs was 153 per female in different sex ratios and the hatchability was 86. 9%. The infection rate of the inoculated larvae was 92.9% and the recovery rate was 82. 1%. The toxicity of the virus preparation reduced by 1% after storage 2 years at - 18 ℃ to 3 ℃.