心理干预对大鼠颞下颌关节病防治作用的实验研究

Experimental study about the effect of mental intervention on controlling temporomandibular joint diseasein rats

目的通过观察心理干预后心理应激状态下大鼠颞下颌关节的相关变化，探讨心理干预在颞下颌关节疾病防治过程中的作用。方法应用交流箱心理应激动物模型对大鼠进行心理应激。分别在应激前后应用抗焦虑药物和去除应激源。用ELISA法和RT-PCR检测各组大鼠颞下颌关节髁突软骨细胞内白细胞介素-1（IL．1）和肿瘤坏死因子（TNF-α0的表达变化。结果PCR结果显示：IL-1βmRNA在应激后第1周表达最强，第3周时减弱，第5周时达到正常水平；TNF-αmRNA在应激后第1周表达最强，第3周后逐渐达到正常水平。Elisa结果显示：抗焦虑药物组IL-10D值（分别为0．1074±0．024，0．101±0．005，0．088±0．010）、去除应激源恢复组IL-10D值（分别为0．090±0．016，0．088±0．005，0．089±0．011）和肿瘤坏死因子的OD值（分别为0．095±0．006，0．077±0．007，0．069±0．009；0．079±0．010，0．075±0．009，0．079±0．012）与对照组（分别为0．087±0．004，0．090±0．009，0．089±0．010；0．074±0．008，0．069±0．015，0．068±0．011）差异不显著（P〉0．05）；抗焦虑药物组、去除应激源恢复组IL-1和TNF-α仅的OD值与心理应激组（分别为0．282±0．045，0．226±0．021，0．092±0．002；0．164±0．009，0．123±0．013，0．091±0．006）有显著差异（P〈0．05）。结论应用抗焦虑药物和去除应激源能有效降低心理应激对大鼠颞下颌关节的影响。

Objective To investigate the role of psychological intervention in the prevention of the temporomandibular joint disease （TMD）, through the observation of the relative changes in the rat TMJ under psychological stress after psychological intervention. Methods The rat model of communication box was built to exert the psychological stress. The antianxiety agent was applied before stress, and the stressor was removed after stress. The expression of the proinflammatory cytokines IL-1 and TNF-α in the mandibular condylar chondrocytes in rat TMJ was detected by ELISA and RT-PCR. Results The RT-PCR results showed that the expression of IL-1 mR- NA increased into the peak in the l st week, weakened in the 3rd week, and returned to normal in the 5th week, while the TNF-oLmRNA peaked in the 1st week, returned to normal in the 3rd week. The ELISA results showed that there was no significant difference of the OD value of the serum IL-land TNF-α （0. 095 ± 0. 006,0. 077 ± 0. 007,0. 069± 0. 009 ;0.079 ±0. 010,0.075 ± 0. 009,0. 079± 0. 012 ） in the antianxiety agent group （ 0. 107 ±0. 024,0. 101 ±0O. 005,0. 088 ±0.010 ） and the stressor removal group （ 0. 090 ± 0. 016,0. 088 ± 0. 005,0. 089 ±0.011 ） , compared with the control group （ 0. 087 ± 0. 004,0. 090± 0. 009,0. 089 ± 0.010 ;0.074±0. 008, 0. 069 ±015,0. 068±0.011 ） （P 〉 0. 05 ）, while significant differences were observed when compared with the psychological stress group（0. 282 ±0.045,0. 226±0.021,0. 092 ± 0. 002 ;0. 164 ±0. 009,0. 123± 0.013,0. 091 ±0. 006 ） （P 〈 0.05 ）. Conclusion Application of the antianxiety agent and stressor removal could effectively counter the influence of psychological stress to TMJ, which provides good experience for the clinical prevention of TMD.