全反式维甲酸对颈动脉内膜损伤后MMP-2和TIMP-2表达的影响

Effect of all-transretinoic acid on expression of MMP-2 and TIMP-2 in injured carotid artery of rabbits

目的观察全反式维甲酸(ATRA)对血管损伤后细胞外基质以及TIMP-2和MMP-2表达的影响。方法 36只新西兰大白兔随机分为正常对照组、模型组和ATRA干预组。分析动脉内膜增生、管腔狭窄和细胞外基质沉积情况,Western blot方法检测肺组织MMP-2和TIMP-2表达。结果内膜损伤后ATRA干预可抑制MMP-2蛋白表达,促进TIMP-2蛋白表达;ATRA干预组的内膜增生程度轻于模型组,血管新生内膜中细胞外基质沉积也明显减少(P<0.05)。结论 ATRA在血管内膜损伤早期能促进TIMP-2表达、抑制MMP-2表达,抑制细胞外基质沉积和平滑肌细胞迁移,抑制内膜过度增生。

Objective To observe the effect of all-transretinoic acid（ATRA） on expression of MMP-2 and TIMP-2 in injured carotid artery of rabbits. Methods Thirty-six rabbits were randomly allocated into normal control group, model group and ATRA treatment group. Proliferation of tunica intima, lumen stricture and deposition of extracellular matrix in injured carotid artery of rabbits were analyzed. Expression of MMP-2 and TIMP-2 in lung tissues was detected by Western blot. Results Treatment of ATRA could inhibit the expression of MMP-2 and increase the expression of TIMP-2 after injury of carotid artery tunica intima in rabbits. The proliferation of carotid artery tunica intima was milder and the deposition of extracellular matrix in tunica intima of newly formed blood vessels was significantly less in ATRA treatment group than in model group（P〈0.05）. Conclusion ATRA can promote the expression of TIMP-2, inhibit the deposition of extracellular matrix, over proliferation of tunica intima, and migration of smooth muscle cells after early injury of carotid artery tunica intima in rabbits.