非酒精性脂肪肝患者血浆HO-1及HO-CO表达的研究

Expression of HO-1 and endogenous CO in plasma in patient with nonalcoholic fatty liver disease

目的探讨血红素氧合酶—内源性一氧化碳(HO-CO)系统与非酒精性脂肪肝发生发展的关系。方法根据临床症状、肝功能、B超诊断非酒精性脂肪肝,分为非酒精性脂肪肝组(ND组)和健康对照组(NC组),分别采用ELISA法检测各组血浆HO-1水平,采用比色法检测各组血浆HO-CO水平,并与健康对照组进行统计学分析。结果与NC组比较,ND组的BMI、甘油三酯、胆固醇、肝功能均升高,差异有统计学意义(P<0.05);血浆HO-1和HO-CO表达亦升高,差异有统计学意义(P<0.05);血浆HO-1与HO-CO表达相关,差异有统计学意义(P<0.05)。血浆HO-1与BMI、甘油三酯、胆固醇呈正相关(P<0.05),HO-CO与BMI、甘油三酯呈正相关(P<0.05)。结论 HO-CO系统参与了非酒精性脂肪肝的病理生理过程,血浆HO-1及HO-CO水平均升高,表明非酒精性脂肪肝发病过程中氧化应激显著。

Objective To explore the relationship between HO-CO system with the pathogenesis and development of nonalcoholic fatty liver disease.Methods The diagnosis of nonalcoholic fatty liver disease according to symptom,hepatic function and B-mode ultrasonic diagnostic equipment.There were two groups,group A：patients of nonalcoholic fatty liver disease,group B：normal control group.The expression of HO-1 was detected by ELISA,and the expression of endogenous CO was detected by colorimeter.Results There was significant difference of BMI,triglyceride,cholesterol,hepatic function,expression of HO-1 and endogenous CO between group A and B（P0.05）.There were correlation between the expression of HO-1 and the expression of endogenous CO,and the expression of HO-1 had positive correlation with BMI,triglyceride,cholesterol,and the expression of endogenous CO had positive correlation with BMI and triglyceride（P0.05）.Conclusion Because the improvement of the expression of HO-1 and endogenous CO can increase oxidative stress,HO-CO system maybe participate in the pathogenesis and development of nonalcoholic fatty liver disease