海带多糖拮抗肾上腺素致血管内膜接触性损伤的作用研究

THE STUDY OF ANTAGONISM OF EPINEPHRINE CONTACT INJURY BY POLYSACCHARIDE FROM LAMINARIA ON THE ENDANGIUM OF RABBITS

目的:研究海带多糖拮抗肾上腺素致血管内膜接触性损伤、减少组织因子(TF)表达的作用。方法:建立肾上腺素致家兔股动脉内膜接触性损伤模型,将家兔分成5组,分别通过插管把生理盐水、肾上腺素-生理盐水液,低剂量、高剂量海带多糖-肾上腺素液和依诺肝素-肾上腺素液注入相应家兔组的游离股动脉中,保留25 min。取与药物接触的股动脉段,常规切片,HE染色,观察血管内膜的完整情况;将切片作TF免疫组化,以血管平均灰度值反映TF在内皮层和内皮下组织的表达情况,灰度值与TF染色成反比。结果:股动脉血管内膜HE染色切片显示,生理盐水组内膜完整、光滑;肾上腺素组内皮层脱落、缺失;海带多糖低剂量组大体完整,内皮局部脱落;海带多糖高剂量组内皮层完整,局部可见与皮下组织分离;依诺肝素组较完整,局部表面粗糙。TF蛋白免疫组化显示,海带多糖低剂量组(68.75±13.21)、海带多糖高剂量组(85.00±7.37)和依诺肝素组(105.44±15.15)平均灰度值均明显高于肾上腺素组(41.12±10.12)(均P<0.01)。结论:海带多糖能够保护血管内膜的完整性,减少TF表达。

Objective：To study the antagonistic effects of polysaccharide from Laminaria on the epinephrine contact injury and the tissue factor （TF） expression on the endangium of rabbits. Methods：To establish a endangium injury model induced by epinephrine on the femoral artery of rabbits. The rabbits were divided into five groups：saline, epinephrine-saline mixture, high, low dose of Laminaria-epinephrine mixture, and enoxaparin-epinephrine mixture was injected respectively to the dissociated femoral artery in the corresponding rabbit groups, and the solution was keeped in the arteries for 25 rain. The drug contact arterial segments were conventional sectioned and stained with HE for the observation of the integrality of arterial endangium. The TF immunohistochemistry was done for the demonstration of TF expression in endothelium and subendothelial tissue with mean gray value which is reverse proportional to TF dying. Results： HE stained slices of femoral artery showed that endangium was intact and smooth in saline group, faulted with missing endothelium section in epinephirne group, intact at large with local missing endothelium in low dose Laminaria group, intact with local separation of endothelium and subendothelial tissue in high dose Laminaria group, intact with local roughness in enoxaparin group. The TF immunohistochemistry showed that mean gray value in low dose Laminaria group（68.75 ±13.21）, high dose Laminaria group（85.00 ± 7. 37） and epinephrine guoup （105.44±15.15）were greater than that in epinephrine group（41.12±10. 12, P 〈0.01）. Conclusion：Polysaccharide from Laminaria can protect the integrality of vessel endthangium and reduce the TF expression.