蛤蚧肽对小鼠免疫功能的调节作用

THE EFFECT OF GEKKO GECKO PEPTIDES ON IMMUNE FUNCTIONS OF IMMUNOSUPPRESSION MICE

目的:观察蛤蚧肽对免疫功能低下小鼠免疫功能的影响。方法:动物分组设正常对照组、模型阴性对照组、左旋咪唑(25mg/kg)阳性对照组、蛤蚧肽三剂量(400,200,100 mg/kg)组,腹腔注射环磷酰胺(CTX)造成小鼠免疫功能低下模型,给药7 d后,应用MTS法测定小鼠脾淋巴细胞增殖反应、自然杀伤细胞(NK)活性、腹腔巨噬细胞杀瘤活性,以半数溶血值HC50法测定血清溶血素的形成。结果:小鼠连续7 d灌胃蛤蚧肽400,200 mg/kg,可对抗CTX 40 mg/kg,连用3 d引起的小鼠脾淋巴细胞增殖指数、NK细胞和腹腔巨噬细胞杀瘤活性下降。连续7 d给蛤蚧肽400,200,100 mg/kg,显著地恢复CTX 15 mg/kg,连用3 d引起的小鼠血清溶血素降低。结论:蛤蚧肽对免疫功能低下小鼠免疫功能具有明显的增强作用。

Objective. To observe the effects of Gekko gecko peptides on immunological regulation of immunosuppression mice. Methods： The immunosuppressed mouse model was established by cyclophosphamide （CTX） injected into abdominal cavity once a day for three days. Immunity of the mice was inhibited in the fourth day of drug withdrawal. Does of CTX was determined according to different test index. Meanwhile, the experimental animal was divided into six groups included the normal control, model negative control, levamisole（25 mg/kg） as positive control, three doses groups （400,200,100 mg/kg）of Gekko gecko peptides. After seventh day of administration the treatment, it was carried out measuring the spleens lymphocyte proliferation, the activity of NK cells, activity of peritoneal macrophages to kill tumor cells by applica- tion MTS method and the production of serum hemolysin by HCs0 method. Results：Gekko gecko peptides at doses of 400,200 mg/kg× 7 d,ig could increase the lymphocyte proliferation index, enhance the activity of both NK cells and peritoneal macrophages to kill tumor in immunosuppressed mice resulted from CTX 40mg/kg× 3 d,ip. Gekko gecko peptides at doses of 400,200,100 mg/kg× 7 d, ig could significantly increase the formation of hemolysin in immunosuppressed mice induced by CTX 15mg/kg×3 d ip. Conclusion：Gekko gecko peptides have an obvious effect of immunological enhancement in the mice of hypoimmunity.