实时荧光定量PCR在植物害虫研究中的应用

Application of Real-time Fluorescence Quantitative PCR on Plant Pests

实时荧光定量PCR技术作为一项新兴技术,以快速、准确定量、便捷等优点广泛应用于科学研究各个领域,越来越受到人们的重视。近年来,随着实时荧光定量PCR技术的发展和深入,大大提高了植物害虫的研究水平。本文结合了最近几年来应用于鉴定和研究植物害虫的情况,同时也介绍了在植物病原线虫和害螨研究中的应用,综合论述了实时荧光定量PCR技术在植物害虫研究中的应用。

As an emerging technology,it has been widely used in different research areas,since it is fast,accurate,and easy use,more and more people pay attention to the real-time fluorescence quantitative PCR.In recent years,with the developing and more detailed of the technology,RQ PCR has greatly improved the level of research on plant pests.In this review,it was summarized and introduced application of real-time fluorescence quantitative PCR on insect pests,pathogenic nematodes and mites.