粗毛淫羊藿的类黄酮异戊烯转移酶基因的克隆与表达分析

Cloning and Expression Analysis of Flavonoid Prenyltransferase-Like Genes in Epimedium acuminatum Franch

药用植物淫羊藿富含异戊烯类黄酮,LC-MS定量分析表明,3～4月份粗毛淫羊藿叶片处于变色期时淫羊藿苷含量最高。通过简并引物和RACE-PCR从粗毛淫羊藿叶片获得异戊烯转移酶同源基因(命名为EaPT1)。序列分析表明,EaPT1与维生素E合成相关的尿黑酸异戊烯转移酶位于同一进化枝,靠近类黄酮异戊烯转移酶。RT-PCR结果显示,EaPT1在叶片和幼茎中表达显著。

The traditional Chinese medicinal plant, Epimedium acuminatum, is remarkably rich in pharmaco-logically active prenylflavonoids. LC-MS analysis indicated that the leaves ofEpimedium acuminatum had the highest icariin content in the color phase （from March to April）. A prenyltransferase-like gene （named EaPT1） was cloned from those leaves with degenerate primers and subsequent rapid amplification of cDNA ends （RACE）. The deduced protein sequence of EaPT1 grouped together with homogentisate phytyltransferases relevant to vitamin E biosynthesis and closely related to the flavonoid prenyltransferases. The EaPT1 transcripts significantly accumulated in leaves and young stems by RT-PCR.