乳康汤对乳腺增生大鼠乳头形态和雌二醇、孕酮受体表达的影响

The Effect of Ru kang tang on Breast Morphology and Expressions of Estrogen and Prolactin Receptors in Breast Hyperplasia Rats

目的探讨乳康汤对乳腺增生模型大鼠乳头形态和血清性激素水平的影响。方法采用苯甲酸雌二醇配合应用少量黄体酮，建立大鼠乳腺增生病模型，研究乳康汤对该模型大鼠乳头形态、组织学和雌激素水平变化的影响，取大鼠第3对乳腺组织，观察乳腺组织病理变化，并采用免疫组化方法观测乳腺组织中雌二醇受体（ER）和孕酮受体（PR）的变化。结果乳康汤均能明显改善模型鼠乳头增生性形态学改变（P〈0．01），能明显降低模型大鼠血清中雌二醇和泌乳素的水平（P〈0．01或P〈0．05）；能明显升高模型大鼠血清中孕酮的水平（P〈0．01或P〈0．05）。结论乳康汤具有抑制乳腺增生模型鼠乳头增生形态的改变；乳康汤抑制乳腺增生；对乳腺增生模型大鼠的治疗作用可能与改善机体内分泌环境有关。

Objectives Effect of Ru kang tang on Serum Sex HormoneLevel of Hyperplasia of Mammary Gland in Rats. Methods Rat breast hyperplasia was established with estradiol benzoate and progesterone to investigate the effects of Ru kang tang on breast morphology, estrogen level and Changesin breast tissue. The 3^rd pair of breast of the rats was taken out and to observe pathological and histological changes.The expressions of estrogen receptor and prolactinp receptor in mammary glandular tissue were analyzed by immuno - histochemistry technique. Results Ru kang tang may have effects of inhibiting breast hyperplasia on model rats;, could decrease the serum levels of Estrogen and Prolactin in rats significantly （P〈0.01, P〈0.05）; could step up the level of Progesterone obviously in rats （P〈0.01, P〈0.05）; Conclusion Ru kang tang may have effects of inhibiting breast hyperplasia on model rats; and Ru kang tang inhibit breast hyperplasia;The therapeutical effectsof prescription on hyperplasia of mammary gland in rats are related to the improvementof endocrine environment.