HPLC-ELSD法测定栀子不同炮制品中栀子苷、藏红花素和藏红花酸的含量

Determination of Geniposide,Crocin and Crocetin in Different Processing Products of Fructus Gardeniae by HPLC-ELSD

目的:采用HPLC-ELSD法测定栀子不同炮制品中栀子苷、藏红花素和藏红花酸的含量。方法:使用蒸发光散射检测器(ELSD)检测器,色谱柱为HyperClone ODS C18柱(250 mm×4.6 mm,5μm),流动相选择甲醇-0.05%磷酸水,梯度洗脱;流速为0.8 mL/min;柱温为30℃;ELSD参数:雾化温度为70℃;气体流量为2.0 L/min。结果:用HPLC-ELSD法测得栀子、炒栀子、半焦栀子和全焦栀子中栀子苷和藏红花素的含量随着炮制程度的加重依次递减,半焦栀子和全焦栀子中藏红花酸的含量较生品略有增加。结论:本法简便、准确、重复性好,可作为栀子不同炮制品质量检测和品质评价的方法。

Objective：To develop a HPLC-ELSD method for determination the contents of geniposide,crocin and crocetin in different processing products of Fructus Gardeniae.Methods：The separation was performed in the HyperClone ODS C18column（250 mm×4.6 mm,5μm）with linear gradient elution using methanol-water and 0.05% phosphoric acid in water,the flowing rate was 0.8 mL/min,the column temperature was 30℃,and the ELSD parameter was as follow：70℃ as atomization temperature and 2.0 L/min as the gas flowing rate.Results：The contents of geniposide and crocin in raw,yellowish,carbocoal and scorched Fructus Gardeniae decreased with the deepening of processing degree.However,the content of crocetin in carbocoal and scorched Fructus Gardeniae increased comparing with the raw one.Conclusion：This is a simple and credible quality control method,and can be used for the quality control and comprehensive evaluation for different processed products of Fructus Gardeniae.