IEC-6细胞迁移药理实验模型建立的研究(英文)

Studies on Cell Migration Model in Intestinal Epithelial Restitution for Pharmacological Research

建立适合肠上皮整复研究的IEC-6细胞迁移药理实验模型,并观察黄芪糖复合物对细胞迁移的影响。方法：设立不同的细胞接种密度、划痕后观察时间、辅助材料Matrigel浓度、血清饥饿处理法、细胞迁移抑制剂DF-MO（二氟甲基鸟氨酸）浓度等以考察模型的建立条件,并在已建立的模型上观察受试药的效果。结果：①细胞宜以4×105/mL接种6孔板;②宜划痕后24 h观察细胞迁移数;③5%Matrigel为适宜浓度;④血清饥饿可造成细胞迁移数明显减少,应使用含血清的培养液。⑤DFMO 2.5~5 mmol/L为抑制细胞迁移适宜浓度。⑥黄芪糖复合物及阳性药精脒能促进细胞迁移。结论：建立了适宜药理实验的IEC-6细胞迁移模型,在此模型上可反映受试药对细胞正常迁移及迁移抑制的药效作用。

Objective：To set up a suitable IEC-6 migration model for pharmacological research and observe the effect of complex polysaccharide from Astragalus membranaceus （Fisch.）Bge.var.mongholicus（Bge.） Hsiao to IEC-6 cell migration.Methods：The main conditions related to the establishment of the model,including the planting density of the cell,the observation time after scratching,the concentration of the auxiliary material Matrigel,the treatment of the serum-starvation,the concentration of α-difluoromethylornithine （DFMO）,an inhibitor of the cell migration,were investigated respectively;and the effects of the tested medicines on the model were observed.Results：4×105cell/mL was the suitable planting density of the cell in the 6-well plate;at the 24th hour after scratching was the appropriate time to count the migrating cells;and the proper concentration of Matrigel was 5%;the serum-starvation could evidently reduce the migrating cells,so the culture medium should contain the serum;2.5 ~5 mmol/L DFMO was proper for inhibition of the cell migration.Complex polysaccharide fromAstragalus membranaceusand spermidine both can promote cell migration.Conclusion：The established model of IEC-6 cell migration was suitable for intestinal epithelial restitution such as the researches on pathophysiological mechanisms is the effects of the medicines on the cell migration.