干姜、辛夷、荜橙茄挥发油对罗通定经皮促渗作用

Enhancing Effect of Volatile Oils of Rhizoma Zingiberis,Flos Magnoliae and Fructus Litseae on Permeation of Rotundine in vitro

目的:研究干姜、辛夷、荜橙茄3种挥发油和氮酮及其两两等量组合后对罗通定的经皮促渗作用。方法:采用改良的Franz扩散池进行小鼠体外经皮渗透实验。以UV法及HPLC法测定罗通定的累积渗透量,筛选对罗通定有最佳促渗作用的挥发油。结果:3种挥发油对罗通定均具有促渗作用,其中5%荜橙茄油、5%干姜油、2.5%荜澄茄油+2.5%氮酮对罗通定的8 h平均累计渗透量(Q8,mg/cm2)分别为:6.0758、6.1148、6.5487;4 h的增渗倍数分别为:1.01、1.00、1.13。结论:2.5%荜澄茄油+2.5%氮酮对罗通定的促渗效果最好。

Objective：To investigate the enhancing effect of volatile oils of Rhizoma Zingiberis（RZ）、Flos Magnoliae（FM）、Fructus Litseae（FL）,Azone and pairwise combinations on the permeation of Rotundine in vitro.Methods：To screen out the volatile oils with the best percutaneous enhancing effect on Rotundine,a test of penetration through rats skin was conducted by using an improved Franz diffusion cell.The accumulative penetration amount of Rotundine was determined by UV and HPLC.Results：All of the 3 volatile oils had enhancing effect on Rotundine permeation.The average accumulative doses of Rotundine for 8 hours（Q8,mg /cm2） of 5% volatile oil of FL、5% volatile oil of,RZ,2.5% FL＋2.5% Azone were 6.0758、6.1148、6.5487,the enhancement ratios of 4 hours were 1.01,1.00,1.13 respectively.Conclusion：2.5% FL＋2.5% Azone had the best effect of percutaneous enhancing on Rotundine.