响应面法优化羊肚菌胞外多糖的发酵条件

Optimization of Culture Conditions for Production of Extracellular Polysaccharides by Morchella esculenta

目的:为获得更多的羊肚菌胞外多糖,探索有利于羊肚菌产生胞外多糖的最佳发酵条件。方法:以羊肚菌GIM5.69为研究对象,首先对影响羊肚菌发酵产生胞外多糖的诸多外界因素进行Plackett-Burman设计试验,筛选出主要影响因素;然后采取响应面法,用Box-Behnken设计试验探寻各影响因素的最佳水平。结果:羊肚菌胞外多糖的最佳液体发酵培养条件为:蔗糖3.98%,培养时间5.98 d,转速217.44 r/min。此条件下的验证试验表明,此时胞外多糖的得率为53.04%,与理论值基本吻合,误差仅为1.64%。结论:该方法对羊肚菌多糖的开发与应用有重要意义。

Objective：To optimize the conditions for exopolysaccharides（EPS）production by Morchella esculenta GIM 5.69.Methods：A Plackett-Burman design was used to evaluate the effects of variables factors The optimal combined conditions for maximum polysaccharide production were further optimized by response surface methodology.Results：The optimal fermentation conditions were determined as follows：glucose 30%,cultivation time 5.98 d,rotary speed 217.44 r/min.The average yield of polysaccharide validation experiments was 53.04%,the relative error was 1.64%.Conclusion： This research has the vital significance regarding the Morchella esculenta polysaccharide function of the product devolopment and the application.