超临界CO_2萃取老挝产鸡蛋花挥发油的研究

Research of the Essential Oil of Plumeria rubra var. actifolia from Laos by Supercritical Carbon Dioxide Extraction

首次采用正交试验设计对老挝产鸡蛋花挥发油的超临界CO2萃取工艺提取进行优化,并与水蒸气蒸馏法进行比较,得最佳萃取条件为:萃取压力25 MPa,萃取温度45℃;分离釜I压力12 MPa,温度55℃;分离釜Ⅱ压力6 MPa,温度30℃,在此工艺条件下的收率为5.8927%。应用气相色谱-质谱联用技术对鸡蛋花挥发油化学成分进行鉴定,用归一化法测定其相对含量。超临界萃取物主要成分有橙花叔醇、柳酸苄酯、邻苯二甲酸二异丁酯等53种化学成分,其中邻苯二甲酸二异丁酯含量高达66.11%,与水蒸气蒸馏法有较大差异。

The orthogonal test and the supercritical carbon dioxide fluid extraction were used for optimizing the extraction of the essential oil from Plumeria rubra var.actifolia for the first time.Compared with the steam distillation,the optimal operation parameter of extraction was as follows：extraction pressure 25 MPa,extraction temperature 45 ℃;separatorⅠpressure 12 MPa,separator Ⅰtemperature 55 ℃;separatorⅡpressure 6 MPa,separatorⅡtemperature 30 ℃.Under this condition the yield of the essential oil was 5.8927%.The components were separated and identified by GC-MS.53 components of Plumeria rubra var.actifolia measured by SFE method were identified and determined by normalization method.The main components were 1,6,10-dodecatrien-3-ol,3,7,11-trimethyl,benzoic acid,2-hydroxy-,phenylmethyl ester,1,2-benzenedicarboxylic acid,bis（2-methylpropyl）ester,etc..1,2-Benzenedicarboxylic acid,bis（2-methylpropyl）este.took up 66.11% of the total amount,and there was much difference of the results from SD method.