磁微粒酶联免疫吸附法检测人体尿液中日本血吸虫抗体

Detection of urine antibodies to Schistosoma japonicum by magnetic particle affinity immunoassay

目的应用磁微粒酶联免疫吸附法(MPAIA)检测日本血吸虫病患者尿液中日本血吸虫特异性抗体,探索血吸虫病新的无创性免疫诊断方法。方法采用MPAIA对158例日本血吸虫病患者和100例健康人的尿液进行检测,并同步进行血清检测,评价其检测效果。结果 MPAIA尿液检测加样量确定为10μl未特殊处理原尿,尿液与血清阳性检出率分别为48.10%(76/158)和88.61%(140/158),两者差异有统计学意义(χ2=60.24,P<0.05),尿液及血清检测特异度均为100%。结论 MPAIA可用于检测日本血吸虫病患者尿液中抗日本血吸虫抗体,但还须对影响其阳性检出率的多种因素进行系统研究,以提高其敏感性。

Objective To explore a non-invasive method for detection of urine antibodies to Schistosoma japonicum. Methods The urine antibodies to S. japonicum were detected by magnetic particle affinity immunoassay （MPAIA） in 158 cases of schistosomiasis japonica and 100 health persons, and their serum antibodies to S. japonicum were also detected at the same time. Results The sample of urine by MPAIA was 10 μl original urine without any special treatment. The positive rate of urine and serum were 48.10%（76/158）and 88.61%（140/158）, respectively. There was difference between the performance of two methods （[χ2]=60.24,P〈0.05）. However, both of their specificity were 100% （100/100）. Conclusion MPAIA is viable for detection of urine antibodies to S. japonicum, but its sensitivity should be improved.