表面修饰磺化壳聚糖的磁性纳米颗粒富集儿茶酚胺的初步研究

A Preliminary Study on the Conjugation of Catecholamines on Magnetic Nanoparticles Coated with Sulfonated Chitosan

文章合成了表面修饰磺化壳聚糖的新型氧化铁磁性纳米颗粒(HSO3-CS-DMSA@MNPS),并考察了该纳米颗粒富集儿茶酚胺类神经递质的效率,并筛选洗脱剂和优化洗脱条件.该纳米颗粒对去甲肾上腺素、肾上腺素和多巴胺的富集率分别为97.3±3.1%、95.2±2.7%和93.9±4.3%;以60%:40%(体积比)的甲醇水溶液(pH=3.0)为洗脱剂,洗脱效率分别为63.6±7.1%4、8.2±5.0%和54.2±6.7%.

In the present study,a novel magnetic nanoparticle coated with sulfonated complex（HSO3-CS-DMSA） of meso-2,3-dimercaptosuccinic acid（DMSA） and chitosan（CS） was synthesized.The conjugation of catecholamines（CAs） on the nanoparticle was investigated and the elution reagent for catecholamines was screened.CAs＇ conjugation efficiency on the magnetic nanoparticles was 97.3±3.1 % for norepinephrine,95.2±2.7 % for epinephrine and 93.9±4.3 % for dopamine.When being washed with 60%（V： V） methanol-water mixtures（pH=3.0）,the elution efficiency was 63.6±7.1% for norepinephrine,48.2±5.0% for epinephrine and 54.2±6.7 % for dopamine.