摘要
目的探讨不同结构的丝素蛋白支架材料对小鼠胚胎成纤维细胞(NIH-3T3)生长繁殖性能的影响。方法将NIH-3T3接种在丝素蛋白纳米纤维膜和浇铸膜上进行体外培养,并设空白对照组。通过MTT法、HE染色、扫描电镜来分析不同结构丝素蛋白膜的生物相容性。结果增殖培养4天时实验组的OD值与对照组相比,有显著性差异(P<0.05);增殖培养7天时实验组与空白组相比无显著性差异(P>0.05),而对照组与空白组相比有显著性差异(P<0.05)。HE染色、扫描电镜显示NIH-3T3细胞在纳米纤维膜上比在浇铸膜上有更好的生长和连接状态。结论与丝素蛋白浇铸膜相比,NIH-3T3细胞在纳米纤维膜上表现出更好的增殖能力,无明显细胞毒性表现;丝素蛋白纳米纤维膜具有良好的生物相容性,是皮肤组织工程的良好载体。
Objective To explore the functions of different silk fibroin membranes to the growth and the proliferation of mouse embryo fibroblasts （NIH-3T3）. Methods NIH-3T3 cells were cultured on silk fihroin nanofibrous and casting membranes. The biocompatibility of silk fibroin membranes with different structures were studied by MTT, HE staining, scanning electron microscope methods Results There was a significant difference （P 〈 0.05） between the experimental group and the control when cellshave grown for 4 days. NIH-3T3 cells on silk fibroin nanofibrous membrane displayed better proliferation ability compared with casting membrane. Conclusion Silk fibroin nanofibrous membrane has good bioeompatibility and will be a good earrier for skin tissue engineering.
出处
《健康研究》
CAS
2011年第3期169-171,174,F0003,共5页
Health Research
基金
973计划前期研究专项课题(2008CB617506)
杭州市卫生科技计划项目(2009A013)
杭州市科技发展计划项目(20090833B24)
