Oligo基因芯片筛选视网膜慢性高眼压损伤差异表达基因的初步数据分析

Differential expression in retina of chronic high intraocular pressure SD rats by large-scale Oligo microarray

目的采用35 000点的高密度Oligo基因芯片对大鼠慢性高眼压导致视网膜损害的6个时间点(7、35、60、90、180、360 d)的基因表达情况加以研究,探讨慢性高眼压影响下视网膜基因表达改变的情况。方法将基因分成"活动"基因和"差异"基因:在"活动"基因中,要求6个时间点中最大Ratio值/最小Ratio值的比值大于2。将差异基因再分为高、低表达及过表达基因,每一类基因按照其生物学功能进行功能检索、分组。对与轴突生长、导向、突触形成相关基因的功能进行初步探讨。结果差异表达基因1 692个:高表达的基因719个(Ratio≥2),包括6个过表达基因(Ratio≥7);低表达的基因973个(Ratio≤0.5)。719个高表达基因中,功能完全未知者占54%,其余功能已知或部分已知者共分为14组,在损伤过程中所占比例最大的是代谢和物质转运组。轴突生长、导向及突触形成相关的基因,抑制轴突生长的基因及神经元的迁移、轴突的生长相关基因在损伤过程中均有呈高表达。结论 建立了大鼠慢性高眼压损伤视网膜差异基因表达库,差异表达基因在慢性高眼压视网膜损害中可能有重要的意义。

Objective To determine the differentially expressed genes in the retina of SD rats with chronic high intraocular pressure（IOP） by a large-scale Oligo microarray.Methods After the high IOP SD rat model was established,the rats were sacrificed in 7,35,60,90,180 and 360 d respectively,and their retina was resected for total RNA.After large-scale Oligo microarray,the obtained genes were identified as ＂active＂ or ＂differentiated＂ for the subsequent analysis.Active genes were those which were expressed and had no obvious change in all above-mentioned 6 time points.＂Differentiated＂ gene must be ＂active＂,and the difference between the maximum and minimum of the 6 time points was greater than 2-fold change（≥2 or ≤0.5）.Among the total 35 000 genes on the gene chip,the differential genes were divided to higher expressed（Ratio≥2） and lower expressed genes（Ratio≤0.5）,and were further categorized according to their functions.Some important genes facilitating to axon growth,orientation and guidance were further analyzed.Results There were 1 692 genes identified as differentiated,with 719 genes of high expression（Ratio ≥2）,including 6 of over-expression（Ratio ≥7）,and with 973 genes of low expression（Ratio≤0.5）.Among the highly expressed 719 genes,there were 54% with no idea about their functions,and the left with known or partially known functions were categorized into 14 groups.During the retinal injury repairing,the genes related to protein synthesis,material transduction and metabolism were most significant,and the genes facilitating to axon growth,orientation and guidance,related to suppress axon growth and neuron migration were also significant.Conclusion A differential genes bank of chronic high IOP injury of SD rat retina is established,and these differential genes might play vital roles in the retinal injury induced by high IOP.