三种泌尿生殖系统支原体感染检测方法的价值比较

COMPARISON OF THREE DETECTIONS OF MYCOPLASMA GENITALIUM INFECTION

目的探讨液体培养法、固体培养法及荧光定量多聚酶链式反应(FQ-PCR)平行检测解脲脲原体(Uu)和人型支原的优劣及临床价值。方法用液体培养法、固体培养法及FQ-PCR平行检测200例临床标本,根据结果作受试者工作曲线(ROC)并给予评价。结果三种不同方法的总检出率比较差异无统计学意义;FQ-PCR法灵敏度及特异度高,ROC曲线下面积最大,其诊断价值高于另两种检测方法。结论液体培养法可作为泌尿生殖系统支原体感染的初筛,但需固体培养进一步确诊,FQ-PCR检测迅速准确,若配合药敏试验,可为临床治疗提供及时、有价值的指导。

Objective To explore the clinic effect and value of three detections of mycoplasma genitalium infection.Method Clinical samples from 200 subjects were received three detections respectively.Receiver operating characteristic curve（ROC） was drawn by SPSS. Result The detectable rate among detections showed no significant difference.ROC indicated higher sensitivity and specificity and diagnostic value of FQ-PCR than other two methods. Conclusion Broth method could be applied as preliminary screening followed by diagnostic plate method.FQ-PCR,due to its rapidity and accuracy,conjugated with drug sensitivity test,would become a promising measure for detection of mycoplasma genitalium infection.