EGCG对TF/Ⅶa/PAR2促进SW620细胞增殖与迁移的干预作用

Effects of epigallocatechin-3-gallate on TF/Ⅶa/PAR2-enhanced proliferation and migration of SW620 cells

目的探讨表没食子儿茶素没食子酸酯(EGCG)对组织因子(TF)/凝血因子Ⅶa/蛋白酶激活受体(PAR)2促进结肠癌细胞株SW620细胞增殖与迁移的干预作用。方法用不同浓度EGCG、蛋白酶激活受体2激动剂(PAR2-AP)、Ⅶa刺激SW620细胞,采用MTT法t、ranswell法分别检测细胞增殖及迁移能力,实时定量PCR检测细胞TF及半胱天冬氨酸蛋白酶(Caspase)7 mRNA表达,发色底物法与Western blot分别检测TF活性、Caspase-7蛋白表达。结果与PAR2-AP或Ⅶa单独处理相比,EGCG+PAR2-AP、EGCG+Ⅶa对SW620细胞增殖、迁移的促进作用明显降低,TF mRNA表达及活性下降,Caspase-7 mRNA及蛋白表达上调(P<0.05)。结论 EGCG可干预SW620细胞TF和Caspase-7的表达,抑制TF/Ⅶa/PAR2对细胞增殖与迁移的促进作用。

Objective To investigate the interference of epigallocatechin-3-gallate（EGCG） on tissue factor（TF）/blood coagulation factor Ⅶa/protease-activated receptor（PAR）2-promoted proliferation and migration of colon cancer SW620 cells.Methods The SW620 cells were treated with different concentrations of EGCG,PAR2 agonist（PAR2-AP） or Ⅶa,and the cell proliferation and migration were analyzed by MTT method and transwell assay,respectively.The mRNA expressions of TF and cystein asparate proteinase（Caspase）-7 were measured by RT-PCR,and the protein expressions of TF and Caspase-7 were detected by chromogenic substrate assays and Western blot,respectively.Results Compared with PAR2-AP or Ⅶa alone,the cell proliferation,cell migration,expression of TF mRNA and TF activity were all inhibited,whereas the expressions of Caspase-7 mRNA and protein were increased after treated with EGCG＋PAR2-AP or EGCG＋Ⅶa（P〈0.05）.Conclusion EGCG can affect the expressions of TF and Caspase-7 in SW620 cells and suppress TF/Ⅶa/PAR2-promoted cell proliferation and cell migration.