胶质瘤中CDK4和RASSF1A表达的相关性及意义

Correlation of CDK4 and RASSF1A expressions and their significance in glioma

目的研究细胞周期蛋白依赖性激酶4(CDK4)和RAS家族相关基因1A(RASSF1A)在脑胶质瘤中的表达情况及相关性。方法采用免疫组织化学SP法,检测70例脑胶质瘤患者、5例非正常死亡脑组织中CDK4和RASSF1A的表达,分析其相关性及与胶质瘤病理分级的关系。结果 CDK4和RASSF1A在不同级别胶质瘤中表达有统计学差异(P<0.05),CDK4与胶质瘤的病理分级呈正相关(rs=0.611,P<0.05),RASSF1A与胶质瘤的病理分级呈负相关(rs=-0.690,P<0.05);CDK4与RASSF1A在脑胶质瘤中的表达呈负相关(rs=-0.676,P<0.05)。结论 CDK4是胶质瘤的正性调节因子,RASSF1A是胶质瘤的负性调节因子,两者参与胶质瘤细胞的增殖和浸润。

Objective To investigate the expressions of cyclin-dependent kinase 4（CDK4） and RAS association domain family 1A（RASSF1A） and their correlation in human gliomas.MethodsImmunohistochemical SP stainning was used to examine the expressions of CDK4 and RASSF1A in 70 human glioma specimens and 5 normal human brain tissue specimens.The relationships among the expressions of CDK4 and RASSF1A in the pathological grade in human gliomas and clinical pathologic characters were analyzed.Results There were significant differences of CDK4 and RASSF1A expressions in different grades of gliomas（P〈0.05）.CDK4 was positively correlated to the pathological grades of gliomas（rs=0.611,P〈0.05）.A negatively correlation was found in the expression of RASSF1A and the grade of gliomas（rs=-0.690,P〈0.05）.The expression of CDK4 was negatively correlated with RASSF1A（rs=-0.621,P〈0.05）.Conclusion CDK4 is a possitive regulator and RASSF1A is a negative regulator for the development of glioma,both of which are involved in the proliferation and invasion of glioma.