血必净联合连翘注射液对脓毒症大鼠影响的肝基因芯片研究

Effects of Xue-Bi-Jing combined with forsythia suspense on the liver gene expression levels of rats with sepsis model

目的探讨血必净联合连翘注射液对脓毒症大鼠基因表达的影响。方法将120只大鼠随机分为假手术组、模型组、泰能组、血必净＋连翘组，每组30只，采用盲肠结扎穿孔术（CLP）复制脓毒症模型。泰能组腹腔注射泰能0．18g／kg；血必净＋连翘组腹腔注射泰能0．18g／kg、血必净注射液10ml／kg及连翘注射液3．9g／kg，每日2次；假手术组和模型组腹腔给予生理盐水10ml／kg；观察各组大鼠48和72h存活率，应用BiostarR-40s芯片检测各组大鼠肝基因表达，通过美国国立生物技术信息中心（NCBI）数据库查询基因功能并加以分类。结果血必净＋连翘组大鼠48h和72h的存活率分别为83.3％和76．7％，明显高于模型组（30．0％和16．7％）与泰能组（60．0％和33．3％），差异均有统计学意义（P〈0．01）。模型组／假手术组共筛选出305条差异基因，其中上调159条（已知基因109条），下调基因146条（已知基因89条）；泰能组／模型组筛选出差异基因386条，其中上调206条（已知基因156条），下调基因180条（已知基因118条）；血必净＋连翘组／模型组筛选出差异基因342条，其中上调102条（已知基因75条），下调基因240条（已知基因120条）。模型组／假手术组上调而血必净＋连翘组／模型组基因下调的已知基因共有24条基因；模型组／假手术组下调而血必净＋连翘组／模型组基因上调的已知基因共有16条基因。结论血必净＋连翘能降低脓毒症大鼠的死亡率，其作用可能与调控相关基因的表达有关。

Objective to study the effects of ＂XUE BI JING plus LIANQIAO＂ injection on gene expression levels of rats with sepsis model. Methods One hundred and twenty rats were randomly divided into sham operation group, sepsis model group, Te-neng group and ＂XUE BI JING plus forsythia suspense＂ group. The sepsis model of rats was prepared by ＂CLP＂ method. Tai neng group was treated by peritoneal injection Imipenem/ Cilastatin （0.18 g/kg）;＂XUE BI JING plus LIANQIAO＂ group was treated by peritoneal injection Imipenem/ Cilastatin（0.18 g/kg） plus ＂xue-bi-jing＂（ 10 ml/kg）and ＂liang ge san＂（ 18 g/200 g） by intragastrie administration 2 times a day; the sham operation group and model group were treated by peritoneal injection of normal saline （ 10 ml/kg）.The survival rates at 48h and 72h were observed for all groups. The gene expression levels of livers in all groups were detected by BiostarR-40s chip. The NCBI database was used to inquest Gene function and class. Results The survival rates at 48h and 72h in ＂XUE BI JING＋ forsythia suspense＂ group were 83.3% and 76.7% which were significantly higher than those （30.0% and 16.7% ） in sepsis model group and those （60.0% and 33.3% ） in Te-neng group （P〈0.01）. Model group/control group have 305 differential expression genes with 159 up-regulation genes and 146 down-regulation genes. Tai-neng group/ model group have 386 differential expression genes with 206 up-regulation genes and 180 down-regulation genes. ＂XUE BI JING plus forsythia suspense＂group/model group have 342 differential expression genes with 102 up-regulation genes and 240 downregulation genes. The genes with up-regulation in model group/control group and with down-regulation in＂XUE BI JING plus forsythia suspense＂group/model group were 24. The genes with down-regulation in the model group/ sham operation group and with up-regulation in ＂XUE BI JING plus forsythia suspense＂group/model group were 16. Conclusion ＂XUE BI JING plus forsythia suspense＂ can reduce the mortality of rats with sepsis, which could be due to the expression of relative regulation genes.