正己烷对人体血清中髓鞘碱性蛋白的影响

Effects of n-hexane exposure on human serum myelin basic proteins

目的了解正己烷接触对人体血清中髓鞘碱性蛋白（MBP）的影响。方法选取正己烷接触1年以上的工人269名作为接触组，同时选取未接触正己烷的工人104名作为对照组，测定工人尿中2,5-己二酮的含量，并依据WS／T243—2004《职业接触正己烷的生物限值》，将含量超出和未超出生物限值的工人分为高接触组和低接触组，使用酶联免疫吸附法测定工人血清中MBP水平。结果接触组工人尿中2,5-己二酮含量均值为（3．10±1．35）mg／L，对照组尿中2，5-己二酮含量均小于最低检出限（0．5mg／L）。低接触组和高接触组工人血清中MBP表达的水平分别为（1．62±0．23）和（2．43±0．24）μg／L，明显高于对照组[（0．78±0．12）μg／L]，差异均有统计学意义（P〈0．01）。经双变量Pearson相关分析，血清中MBP表达水平与尿中2,5-己二酮的含量呈正相关（r=0．781，P〈0．01）。结论正己烷接触可导致人体血清中MBP表达水平升高，血清中MBP可作为正己烷接触的效应标志物。

Objective To explore the effects of n-hexane on expression of serum myelin proteins （MBP） in workers occupationally exposed to n-hexane. Methods In this study, 269 workers exposed to n- hexane for more than one year and 104 subjects not exposed to n-hexane served as the exposure group and the control group, respectively. The urinary 2,5-hexanedione levels in all subjects were detected. On the basis of urinary 2,5-hexanedione levels, the exposure group was divided into the high exposure sub-group and low exposure sub-group. The serum myelin basic protein （MBP） levels were measured by ELISA kit. Results The mean concentration of urinary 2,5-hexanedione in the exposed group was （ 3.10± 1.35 ） mg/L, The concentration of urinary 2,5-hexanedione in the control group was undetectable. The levels of serum MBP in the high exposure sub-group and low exposure sub-group were （2.43±0.24） and （1.62±0.23） μg/L, respectively, which were significantly higher than that （0.78±0.12） μg/L in the controls （P〈0.01 ）. Pearson correlation analysis showed the positive correlation between serum MBP levels and urinary 2,5-hexanedione levels （r =0.781,P 〈0.01）. Conclusion The results of present study showed that the serum MBP levels of workers occupationally exposed to n-hexane significantly elevated, and the serum MBP can serve as the effective biomarker of n-hexane exposure.