Hsa-miR-9在结肠癌组织中的表达及其临床意义

Expression and Clinical Significance of Hsa-miR-9 in Colon Carcinoma

目的:探讨Hsa-miR-9在结肠癌组织中的表达及其临床意义。方法:收集结肠癌患者手术标本62例,癌旁组织作为正常对照,应用原位杂交及实时荧光定量PCR(real time quantity PCR,qRT-PCR)检测结肠癌组织及癌旁组织中Hsa-miR-9的表达水平,以及Hsa-miR-9表达与结肠癌临床病理参数及临床分期之间的关系。结果:原位杂交检测显示,在62例结肠癌组织中Hsa-miR-9阳性表达率为88.71%,与癌旁对照组相比(17.74%),差异有统计学意义(P<0.01);qRT-PCR检测显示,Hsa-miR-9在结肠癌表达与癌旁对照组相比高(3.77±0.42)倍,差异有统计学意义(P<0.01);Hsa-miR-9的表达随着结肠癌临床分期演进而增加,临床Ⅲ/Ⅳ期、Ⅰ/Ⅱ期结肠癌Hsa-miR-9的表达与癌旁对照组比分别高(4.23±0.18)、(2.24±0.31)倍,各组间比较差异均有显著性(P<0.05);有淋巴结转移的结肠癌Hsa-miR-9的表达比无淋巴结转移者高3.12倍,差异有统计学意义(P<0.01);Hsa-miR-9的表达与分化程度之间差异无统计学意义(P>0.05)。结论:Hsa-miR-9与结肠癌的发生、转移密切相关,其可能成为结肠癌治疗、预后判断的潜在生物学指标。

Objective： To explore the expression and clinical significance of hsa-miR-9 in colon carcinoma. Methods： Tissue samples were collected from 62 cases of colon carcinoma from June 2007 to December 2009, with paracancerous tissues as the normal controls. Hybridization in situ and real-time quantitative polymerase chain reaction were used to detect the hsa-miR-9 expression in those specimens. The relationship between hsa-miR-9 expression and the clinicopathologic parameters was determined. Results： Hy- bridization in situ showed that the positive expression rate of the hsa-miR-9 was 88.71% in colon carcinoma and 17.74% in the paracancerous tissues; the difference was statistically significant （ P 〈 0.01 ）. The real-time quantitative PCR showed that the expression of hsa-miR-9 was （ 3.77 ± 0.42 ） times higher in colon carcinoma than in normal tissue; the differences were statistically significant （ P 〈 0.01 ）. This indicates that the upregulation of hsa-miR-9 expression is associated with advanced clinical stages. It was 4.23 ± 0.18 and 2.24 ± 0.31 times higher than paracancerous tissues, respectively, for stage Ⅲ/Ⅳ and stage Ⅰ/Ⅱ colon carcinoma; the difference was statistically significant in different groups （ P 〈 0.05 ）. The expression of hsa-miR-9 was 3.12 times higher with lymph node metastasis than without lymph node metastasis in colon carcinoma; the difference was statistically significant （ P 〈 0.01 ）. The expression of hsa-miR-9 was not associated with degree of differentiation; the difference was not statistically significant （ P 〉 0.05 ）. Conclusion： Hsa-miR-9 is related to the occurrence and metastasis of colon carcinoma, and it could be a predictive biomarker in the treatment and prognosis of colon carcinoma.